FUSARIUM-ID v.3.0: An Updated, Downloadable Resource for Species Identification.

Species within are of global agricultural, medical, and food/feed safety concern and have been extensively characterized. However, accurate identification of species is challenging and usually requires DNA sequence data. -ID (http://isolate.

Phylogenomic Analysis of a 55.1-kb 19-Gene Dataset Resolves a Monophyletic that Includes the Species Complex.

Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. In 2013, the community voiced near unanimous support for a concept of that represented a clade comprising all agriculturally and clinically important species, including the species complex (FSSC). Subsequently, this concept was challenged in 2015 by one research group who proposed dividing the genus into seven genera, including the FSSC described as members of the genus , with subsequent justification in 2018 based on claims that the 2013 concept of is polyphyletic.

Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH).

Background: is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of that causes high crop loss. Currently, there are no available methods for the microscopic detection of from leaves of impatiens, which may be contributing to the spread of the disease. Fluorescence in situ hybridization (FISH) is a sensitive and robust method that uses sequence-specific, fluorescence-labeled oligonucleotide probes to detect target organisms from the environment.

Analysis of microsatellites from the transcriptome of downy mildew pathogens and their application for characterization of populations.

Downy mildew pathogens affect several economically important crops worldwide but, due to their obligate nature, few genetic resources are available for genomic and population analyses. Draft genomes for emergent downy mildew pathogens such as the oomycete , causal agent of cucurbit downy mildew, have been published and can be used to perform comparative genomic analysis and develop tools such as microsatellites to characterize pathogen population structure. We used bioinformatics to identify 2,738 microsatellites in the predicted transcriptome and evaluate them for transferability to the hop downy mildew pathogen, , since no draft genome is available for this species.