"Washing our hands is a superpower": Parent-child conversations about COVID-19 are longitudinally associated with children's social-emotional adjustment and their conceptualizations of the pandemic.

Families with young school-age children experienced unique challenges during the early months of the COVID-19 pandemic. This longitudinal, mixed-methods study explored links between parent-child conversations early in the COVID-19 pandemic and children's later social-emotional adjustment and conceptualizations of the pandemic. In a socioeconomically diverse (36% low-income) sample of 183 families, parents (94% women, 6% men) reported on the content of their conversations about the COVID-19 pandemic in Spring 2020 with their young school-age children (81% White, 8% Hispanic/Latine, 2% Asian/Pacific Islander, 1% Black, 8% multiracial/other; 46% girls, 54% boys, = 7.

Development of a convenient competitive ELISA for the detection of the free and protein-bound nonhuman galactosyl-α-(1,3)-galactose epitope based on highly specific chicken single-chain antibody variable-region fragments.

The presence of the nonhuman galactosyl-α-(1,3)-galactose (Gal-α-(1,3)-Gal) carbohydrate epitope on a number of recombinant therapeutic proteins has recently been reported, renewing interest in this immunogenic carbohydrate epitope. It is well-known that this motif is the primary contributing factor in hyperacute rejection of porcine organ xenograft, due to the existence of natural antibodies against this epitope in human serum. Though the number of epitopes on recombinant glycoproteins may be low when compared directly to whole tissue, circulating anti-Gal-α-R immunoglobulins can still induce anaphylaxis.

Rapid, transient, and proportional activation of σ(B) in response to osmotic stress in Listeria monocytogenes.

The osmotic activation of sigma B (σ(B)) in Listeria monocytogenes was studied by monitoring expression of four known σ(B)-dependent genes, opuCA, lmo2230, lmo2085, and sigB. Activation was found to be rapid, transient, and proportional to the magnitude of the osmotic stress applied, features that underpin the adaptability of this pathogen.