Alveolar macrophage transcriptomic profiling in COPD shows major lipid metabolism changes.

Background: Immune cells play a major role in the pathogenesis of COPD. Changes in the distribution and cellular functions of major immune cells, such as alveolar macrophages (AMs) and neutrophils are well known; however, their transcriptional reprogramming and contribution to the pathophysiology of COPD are still not fully understood.

Method: To determine changes in transcriptional reprogramming and lipid metabolism in the major immune cell type within bronchoalveolar lavage fluid, we analysed whole transcriptomes and lipidomes of sorted CD45LinHLA-DRCD66bAutofluorescence AMs from controls and COPD patients.

Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases.

Human monocytes are divided in three major populations; classical (CD14CD16), non-classical (CD14CD16), and intermediate (CD14CD16). Each of these subsets is distinguished from each other by the expression of distinct surface markers and by their functions in homeostasis and disease. In this review, we discuss the most up-to-date phenotypic classification of human monocytes that has been greatly aided by the application of novel single-cell transcriptomic and mass cytometry technologies.