The Mag1 and Tpa1 proteins from budding yeast () have both been reported to repair alkylation damage in DNA. Mag1 initiates the base excision repair pathway by removing alkylated bases from DNA, and Tpa1 has been proposed to directly repair alkylated bases as does the prototypical oxidative dealkylase AlkB from However, we found that repair of methyl methanesulfonate (MMS)-induced alkylation damage in DNA involves Mag1 but not Tpa1. We observed that yeast strains without are no more sensitive to MMS than WT yeast, whereas -deficient yeast are ∼500-fold more sensitive to MMS.
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