Publications by authors named "Emily M Blaum"
Article Synopsis
- Image-guided cryoablation is a treatment for cancer that freezes tumors to help fight them.
- Researchers believed that this treatment could help the immune system work better against stubborn tumors that didn't respond to other therapies.
- In a study with 17 patients, cryoablation was found to be safe and helped improve tumor responses in some patients, showing promise for this combined treatment approach.
Article Synopsis
- The study examines how variations in tumor microenvironments (TMEs) affect cancer progression by analyzing 52 head and neck squamous cell carcinomas.
- It identifies macrophage polarity—determined by CS expression—as a significant factor for prognosis, rather than traditional M1 and M2 classifications.
- The findings indicate that TMEs create organized networks of pro- and antitumor responses, emphasizing that CS macrophage polarity could simplify understanding complex cancer behaviors across different types of tumors.
Article Synopsis
- Long-read RNA-sequencing methods can capture full transcript isoforms but traditionally have low throughput*. -
- The new technique, multiplexed arrays isoform sequencing (MAS-ISO-seq), enhances this by combining cDNAs for more efficient long-read sequencing, boosting throughput by over 15 times*. -
- In experiments with tumor-infiltrating T cells, MAS-ISO-seq led to a significant increase (12- to 32-fold) in the identification of differentially spliced genes*.
Mechanisms of neutrophil involvement in severe coronavirus disease 2019 (COVID-19) remain incompletely understood. Here, we collect longitudinal blood samples from 306 hospitalized COVID-19 patients and 86 controls and perform bulk RNA sequencing of enriched neutrophils, plasma proteomics, and high-throughput antibody profiling to investigate relationships between neutrophil states and disease severity. We identify dynamic switches between six distinct neutrophil subtypes.
View Article and Find Full Text PDFImmune checkpoint blockade (CPB) improves melanoma outcomes, but many patients still do not respond. Tumor mutational burden (TMB) and tumor-infiltrating T cells are associated with response, and integrative models improve survival prediction. However, integrating immune/tumor-intrinsic features using data from a single assay (DNA/RNA) remains underexplored.
View Article and Find Full Text PDFMultiple studies have identified an association between neutrophils and COVID-19 disease severity; however, the mechanistic basis of this association remains incompletely understood. Here we collected 781 longitudinal blood samples from 306 hospitalized COVID-19 patients, 78 COVID-19 acute respiratory distress syndrome patients, and 8 healthy controls, and performed bulk RNA-sequencing of enriched neutrophils, plasma proteomics, cfDNA measurements and high throughput antibody profiling assays to investigate the relationship between neutrophil states and disease severity or death. We identified dynamic switches between six distinct neutrophil subtypes using non-negative matrix factorization (NMF) clustering.
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