How Gut Microbiome Perturbation Caused by Antibiotic Pre-Treatments Affected the Conjugative Transfer of Antimicrobial Resistance Genes.

The global spread of antimicrobial resistance genes (ARGs) poses a significant threat to public health. While antibiotics effectively treat bacterial infections, they can also induce gut dysbiosis, the severity of which varies depending on the specific antibiotic treatment used. However, it remains unclear how gut dysbiosis affects the mobility and dynamics of ARGs.

Complete genome sequences of a Canadian strain of enteroaggregative (EAEC) with multiple metals and antimicrobial resistance genes isolated from municipal waste-activated sludge.

Enteroaggregative (EAEC) is an emerging food-borne pathogen causing acute or persistent diarrhea in humans. Here, we report the complete genome sequence of a strain of EAEC with multiple metals and antimicrobial resistance genes isolated from a waste-activated sludge collected from a Canadian municipal wastewater treatment plant.

Complete Genome Sequence of a Canadian Strain of Escherichia coli with Multiple Metal and Antimicrobial Resistance Genes That Was Isolated from Municipal Biosolids.

This announcement reports the complete genome sequence of a non-Shiga toxin-producing Escherichia coli strain that was isolated from municipal biosolids collected from a Canadian wastewater treatment plant. This strain contains multiple metal, antimicrobial, and heat resistance genes, as determined by genome sequencing, and could be a useful bacterial model for future studies.

Draft Genomes of 92 Isolates from Warm-Water Shrimps Imported into Canada between 2009 and 2019.

spp. were isolated from raw shrimps imported into Canada (2009 to 2019). A total of 92 isolates with various multidrug resistance profiles were sequenced, including 59 V.

Draft Genomes of 75 Isolates from Retail Mollusks Collected in Canada between 2014 and 2018.

spp. isolated from fresh retail mollusk samples were selected for sequencing based on their antimicrobial resistance burden. The genomes include those for Vibrio alginolyticus ( = 48), ( = 15), V.

Draft Genomes of 83 Isolates from Fresh Canadian Mollusks.

A subset of spp. isolated from fresh Canadian mollusks (2014 to 2018) were selected for sequencing based on antimicrobial resistance profiles. The resulting draft genomes include 38 Vibrio alginolyticus, 32 V.

Genomic analysis of human noroviruses using combined Illumina-Nanopore data.

Whole-genome sequence analysis of noroviruses is routinely performed by employing a metagenomic approach. While this methodology has several advantages, such as allowing for the examination of co-infection, it has some limitations, such as the requirement of high viral load to achieve full-length or near full-length genomic sequences. In this study, we used a pre-amplification step to obtain full-length genomic amplicons from 39 Canadian GII isolates, followed by deep sequencing on Illumina and Oxford Nanopore platforms.

Effect of advanced age and vital signs on admission from an ED observation unit.

Objectives: The primary objective was to determine the relationship between advanced age and need for admission from an emergency department (ED) observation unit. The secondary objective was to determine the relationship between initial ED vital signs and admission.

Methods: We conducted a prospective, observational cohort study of ED patients placed in an ED-based observation unit.

Apple skin patterning is associated with differential expression of MYB10.

Background: Some apple (Malus × domestica Borkh.) varieties have attractive striping patterns, a quality attribute that is important for determining apple fruit market acceptance. Most apple cultivars (e.