Methane-Linked Mechanisms of Electron Uptake from Cathodes by Methanosarcina barkeri.

The , a lineage of cytochrome-containing methanogens, have recently been proposed to participate in direct extracellular electron transfer interactions within syntrophic communities. To shed light on this phenomenon, we applied electrochemical techniques to measure electron uptake from cathodes by , which is an important model organism that is genetically tractable and utilizes a wide range of substrates for methanogenesis. Here, we confirm the ability of to perform electron uptake from cathodes and show that this cathodic current is linked to quantitative increases in methane production.

Duty cycling influences current generation in multi-anode environmental microbial fuel cells.

Improving microbial fuel cell (MFC) performance continues to be the subject of research, yet the role of operating conditions, specifically duty cycling, on MFC performance has been modestly addressed. We present a series of studies in which we use a 15-anode environmental MFC to explore how duty cycling (variations in the time an anode is connected) influences cumulative charge, current, and microbial composition. The data reveal particular switching intervals that result in the greatest time-normalized current.

Dynamical fluctuations in dense granular flows.

We have made measurements of force and velocity fluctuations in a variety of dense, gravity-driven granular flows under flow conditions close to the threshold of jamming. The measurements reveal a microscopic state that evolves rapidly from entirely collisional to largely frictional, as the system is taken close to jamming. On coarse-grained time scales, some descriptors of the dynamics-such as the probability distribution of force fluctuations, or the mean friction angle-do not reflect this profound change in the micromechanics of the flow.

Optical traps with geometric aberrations.

We assess the influence of geometric aberrations on the in-plane performance of optical traps by studying the dynamics of trapped colloidal spheres in deliberately distorted holographic optical tweezers. The lateral stiffness of the traps turns out to be insensitive to moderate amounts of coma, astigmatism, and spherical aberration. Moreover holographic aberration correction enables us to compensate inherent shortcomings in the optical train, thereby adaptively improving its performance.