Plasma cell-free DNA chromatin immunoprecipitation profiling depicts phenotypic and clinical heterogeneity in advanced prostate cancer.

Cell phenotype underlies prostate cancer presentation and treatment resistance and can be regulated by epigenomic features. However, the osteotropic tendency of prostate cancer limits access to metastatic tissue, meaning most prior insights into prostate cancer chromatin biology are from preclinical models that do not fully represent disease complexity. Noninvasive chromatin immunoprecipitation of histones in plasma cell-free in humans may enable capture of disparate prostate cancer phenotypes.

"It Takes a Virtual Village" Achieving Magnet Redesignation Amidst the COVID-19 Pandemic: Canada's Magnet Hospital Journey.

This article outlines how a Canadian hospital achieved the American Nursing Credentialing Center Magnet Recognition Program redesignation after participating in a virtual site visit (VSV) appraisal process amidst the COVID-19 pandemic. Within our current COVID-19 landscape, being a resilient Magnet-designated organization is paramount. In this context, the American Nurses Credentialing Center (ANCC) has developed a VSV model that (1) extends the use of audio/video (A/V) conferencing technology to showcase nursing excellence; (2) maintains the integrity of the appraisal process; and (3) ensures the safety and well-being of staff, patients and their care partners, and the appraisers.

Ni-Catalyzed β-Alkylation of Cyclopropanol-Derived Homoenolates.

Metal homoenolates are valuable synthetic intermediates which provide access to β-functionalized ketones. In this report, we disclose a Ni-catalyzed β-alkylation reaction of cyclopropanol-derived homoenolates using redox-active hydroxyphthalimide (NHPI) esters as the alkylating reagents. The reaction is compatible with 1°, 2°, and 3° NHPI esters.

Coupling of pollination services and coffee suitability under climate change.

Climate change will cause geographic range shifts for pollinators and major crops, with global implications for food security and rural livelihoods. However, little is known about the potential for coupled impacts of climate change on pollinators and crops. Coffee production exemplifies this issue, because large losses in areas suitable for coffee production have been projected due to climate change and because coffee production is dependent on bee pollination.

Exploiting post-transcriptional regulation to probe RNA structures in vivo via fluorescence.

While RNA structures have been extensively characterized in vitro, very few techniques exist to probe RNA structures inside cells. Here, we have exploited mechanisms of post-transcriptional regulation to synthesize fluorescence-based probes that assay RNA structures in vivo. Our probing system involves the co-expression of two constructs: (i) a target RNA and (ii) a reporter containing a probe complementary to a region in the target RNA attached to an RBS-sequestering hairpin and fused to a sequence encoding the green fluorescent protein (GFP).