The antimicrobial activity of metal nanoparticles obtained by biogenic routes has been extensively reported. However, their combined use with other antimicrobial formulations, such as essential oils, remains scarcely explored. In this work, a manganese-ferrite/silver nanocomposite (MnFeO/Ag-NC) was synthesized in a two-step procedure: first, MnFeO nanoparticles were produced by a coprecipitation method, followed by biogenic reduction of silver ions using .
View Article and Find Full Text PDFThe possibility of modifying terpene production in plants is a defensive strategy that has been studied in conjunction with their biosynthetic pathways. A biotic factor such as Arbuscular Mycorrhizal Fungi (AMF) could modify terpene production in L. In this work, the enzymatic production of monoterpenes in Superqueli INIA cultivar with two AMF was evaluated via HeadSpace-Gas Chromatography (HS-GC).
View Article and Find Full Text PDF, is a forage found worldwide, but it is negatively impacted by the clover root borer, . Methanolic extraction has been reported for isolating formononetin from vegetal tissues, with an antifeeding effect on . However, this methodology is time-consuming and also extracts other secondary metabolites, whereas enzymatic assays can provide higher specificity.
View Article and Find Full Text PDFPotato waste was processed and used as a sole substrate for simultaneously producing antifungals and biopigments using Streptomyces spp. Out of three different Streptomyces isolates, strain SO6 stood out due to its ability to produce antifungals against economically important fungal phytopathogens and intracellular biopigments using potato waste powders without additional nutrients. This strain also showed the potential to secrete a broad range of enzymes for fermentation of eight sugars that could be involved in potato waste bioconversion.
View Article and Find Full Text PDFAlkaloid profiles from plants collected in Chile were examined by GC-MS to assess their inhibitory activity on acetylcholinesterase (AChE) using and methodologies. The alkaloid extract was roughly separated by column chromatography on silica gel. AChE inhibitory activities from extracts and purified alkaloids were tested by the Ellman method and a molecular docking study was performed to assess the interaction between AChE and purified alkaloids.
View Article and Find Full Text PDFPoplars are frequently attacked by aphids. The differential susceptibility of poplar hybrids to the aphid Chaitophorus leucomelas Koch (Sternorrhyncha: Aphididae) has been described, but the mechanism underlying this pattern is unknown. This work tested the hypothesis that poplar resistance to this aphid is associated with the presence of volatiles and secondary plant compounds that affect host selection and feeding behavior.
View Article and Find Full Text PDFPenicillium janczewskii K. M. Zalessky was isolated as an endophytic fungus from the phloem of the Chilean gymnosperm Prumnopitys andina.
View Article and Find Full Text PDFSeven compounds belonging to different structural skeletons were isolated from Microsphaeropsis olivacea grown in liquid and solid media. The enalin derivative 7-hydroxy-2,4-dimethyl-3(2H)-benzofuranone is reported for the first time, while additional spectroscopic information is provided for the acetates of botrallin and ulocladol. The activity of the isolated compounds was assessed towards the enzyme acetylcholinesterase (AChE) and their cytotoxicity against human lung fibroblasts.
View Article and Find Full Text PDFA rapid in vitro propagation system leading to the formation of shoots, calli, roots, cell suspensions and plantlets was developed for the Andean medicinal plant Fabiana imbricata (Solanaceae). Massive propagation of shoots and roots was achieved by the temporary immersion system (TIS), morphogenesis and maintenance of cell suspensions by standard in vitro culture techniques. Oleanolic acid (OA), rutin, chlorogenic acid (CA) and scopoletin content in aerial parts of wild growing Fabiana imbricata plants as well as in plantlets regenerated in vitro, callus cultures, cell suspensions and biomass, obtained by the TIS system was assessed by HPLC.
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