The voice of the little giants: Arcellinida testate amoebae in environmental DNA-based bioindication, from taxonomy free to haplotypic level.

Bioindication, evaluating biological responses to environmental disturbances, is crucial for assessing the ecological status of an ecosystem. While historical bioindication relied on macroscopic organisms, the introduction of environmental DNA (eDNA) techniques allows the application of protists without the necessity of morphological identification. In this study, we propose a novel bioindication methodology utilizing Arcellinida, a group of top predators among protists, as bioindicators of freshwater ecosystems.

Phylogenetic and phylogeographic evidence for the colonization success of the diplochorous across the Canary Islands.

is the only species of the genus , which forms an early-diverging lineage of Apiaceae, subfamily Apioideae. This species is subendemic to the Canary Islands and one of the most representative species of the coastal environments of this archipelago. displays diplochory, that is, diaspores with two long-distance dispersal (LDD) syndromes.

Performance of commercial growing-finishing pigs fed supplemental isoquinoline alkaloids: a statistical process control analysis.

Background: Statistical Process Control (SPC) is a powerful statistical tool that can be used in animal production to evaluate the evolution of production parameters overtime in response to the implementation of a specific strategy. The aim of this study was to evaluate the effect of supplementing growing-finishing pigs with isoquinoline alkaloids (IQ) on growth performance parameters by using the SPC method. IQ are natural secondary plant metabolites which have been extensively investigated in food animals due to their efficacy in supporting growth performance and the overall health status.

Corrosion Risk to Metal-Based Artefacts in a Scientific and Technical Museum: An Assessment of Environmental and Exhibition Conditions.

Materials such as wood, textiles, or plastics that are part of the exhibition system in museums are known to emit pollutants such as organic acids. Scientific and technical objects that include these materials in their composition can themselves be a potential source of emissions, which, together with inappropriate humidity and temperature conditions, can lead to corrosion of the metallic parts. In this work, we have studied the corrosivity of different locations in two venues of the Spanish National Museum of Science and Technology (MUNCYT).

Immunogenicity, effectiveness and safety of COVID-19 vaccine in older adults living in nursing homes: A real-life study.

Introduction: BNT162b2 (BioNTech and Pfizer) is a nucleoside-modified mRNA vaccine that provides protection against SARS-CoV-2 infection and is generally well tolerated. However, data about its efficacy, immunogenicity and safety in people of old age or with underlying chronic conditions are scarce.

Purpose: To describe BNT162b2 (BioNTech and Pfizer) COVID-19 vaccine immunogenicity, effectiveness and reactogenicity after complete vaccination (two doses), and immunogenicity and reactogenicity after one booster, in elders residing in nursing homes (NH) and healthy NH workers in real-life conditions.

A needle in a haystack: A new metabarcoding approach to survey diversity at the species level of Arcellinida (Amoebozoa: Tubulinea).

Environmental DNA-based diversity studies have increased in popularity with the development of high throughput sequencing technologies. This permits the potential simultaneous retrieval of vast amounts of molecular data from many different organisms and species, thus contributing to a wide range of biological disciplines. Environmental DNA protocols designed for protists often focused on the highly conserved small subunit of the ribosome gene, that does not permit species-level assignments.

Time-calibrated phylogenies reveal mediterranean and pre-mediterranean origin of the thermophilous vegetation of the Canary Islands.

Background And Aims: The Canary Islands have strong floristic affinities with the Mediterranean Basin. One of the most characteristic and diverse vegetation belts of the archipelago is the thermophilous woodland (between 200 and 900 m.a.

Sensor Data Analytics: Challenges and Methods for Data-Intensive Applications.

Sensors have become a key element for the development of the Information Society [...

Analysis of Accelerometer and GPS Data for Cattle Behaviour Identification and Anomalous Events Detection.

In this paper, a method to classify behavioural patterns of cattle on farms is presented. Animals were equipped with low-cost 3-D accelerometers and GPS sensors, embedded in a commercial device attached to the neck. Accelerometer signals were sampled at 10 Hz, and data from each axis was independently processed to extract 108 features in the time and frequency domains.

Taking Particle Tracking into Practice by Novel Software and Screening Approach: Case-Study of Oral Lipid Nanocarriers.

The success on the design of new oral nanocarriers greatly depends on the identification of the best physicochemical properties that would allow their diffusion across the mucus layer that protects the intestinal epithelium. In this context, particle tracking (PT) has arisen in the pharmaceutical field as an excellent tool to evaluate the diffusion of individual particles across the intestinal mucus. In PT, the trajectories of individual particles are characterized by the mean square displacement (), which is used to calculate the coefficient of diffusion () and the anomalous diffusion parameter () as MSD=4Dτα.

Esterified carotenoids are synthesized in petals of carnation (Dianthus caryophyllus) and accumulate in differentiated chromoplasts.

Although yellow and orange petal colors are derived from carotenoids in many plant species, this has not yet been demonstrated for the order Caryophyllales, which includes carnations. Here, we identified a carnation cultivar with pale yellow flowers that accumulated carotenoids in petals. Additionally, some xanthophyll compounds were esterified, as is the case for yellow flowers in other plant species.

Integration of Phenotype and Hormone Data during Adventitious Rooting in Carnation ( L.) Stem Cuttings.

The rooting of stem cuttings is a highly efficient procedure for the vegetative propagation of ornamental plants. In cultivated carnations, an increased auxin level in the stem cutting base produced by active auxin transport from the leaves triggers adventitious root (AR) formation from the cambium. To provide additional insight into the physiological and genetic basis of this complex trait, we studied AR formation in a collection of 159 F lines derived from a cross between two hybrid cultivars ( and ) showing contrasting rooting performances.

Carnation locus contains two chalcone isomerase genes involved in orange flower coloration.

Carnations carrying a recessive gene show accumulation of the yellow pigment chalcononaringenin 2'-glucoside (Ch2'G) in their flowers, whereas those with a dominant gene do accumulation the red pigment, anthocyanin. Although this metabolic alternative at the gene could explain yellow and red flower phenotypes, it does not explain the development of orange flower phenotypes which result from the simultaneous accumulation of both Ch2'G and anthocyanin. The carnation whole genome sequencing project recently revealed that two chalcone isomerase genes are present, one that is consistent with the gene (Dca60979) and another (Dca60978) that had not been characterized.

Repressed expression of a gene for a basic helix-loop-helix protein causes a white flower phenotype in carnation.

In a previous study, two genes responsible for white flower phenotypes in carnation were identified. These genes encoded enzymes involved in anthocyanin synthesis, namely, flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR), and showed reduced expression in the white flower phenotypes. Here, we identify another candidate gene for white phenotype in carnation flowers using an RNA-seq analysis followed by RT-PCR.

Genome sequence of the olive tree, Olea europaea.

Background: The Mediterranean olive tree (Olea europaea subsp. europaea) was one of the first trees to be domesticated and is currently of major agricultural importance in the Mediterranean region as the source of olive oil. The molecular bases underlying the phenotypic differences among domesticated cultivars, or between domesticated olive trees and their wild relatives, remain poorly understood.

Optimization instances for deterministic and stochastic problems on energy efficient investments planning at the building level.

Optimization instances relate to the input and output data stemming from optimization problems in general. Typically, an optimization problem consists of an objective function to be optimized (either minimized or maximized) and a set of constraints. Thus, objective and constraints are jointly a set of equations in the optimization model.

Quantitative Analysis of Adventitious Root Growth Phenotypes in Carnation Stem Cuttings.

Carnation is one of the most important species on the worldwide market of cut flowers. Commercial carnation cultivars are vegetatively propagated from terminal stem cuttings that undergo a rooting and acclimation process. For some of the new cultivars that are being developed by ornamental breeders, poor adventitious root (AR) formation limits its commercial scaling-up, due to a significant increase in the production costs.

Forest restoration in a fog oasis: evidence indicates need for cultural awareness in constructing the reference.

Background: In the Peruvian Coastal Desert, an archipelago of fog oases, locally called lomas, are centers of biodiversity and of past human activity. Fog interception by a tree canopy, dominated by the legume tree tara (Caesalpinia spinosa), enables the occurrence in the Atiquipa lomas (southern Peru) of an environmental island with a diverse flora and high productivity. Although this forest provides essential services to the local population, it has suffered 90% anthropogenic reduction in area.

Plant tissue distribution and chemical inactivation of six carnation viruses.

Carnation mottle virus (CarMV), Carnation etched ring virus (CERV), Carnation vein mottle virus (CVMV), Carnation ringspot virus (CRSV), Carnation Italian ringspot virus (CIRV) and Carnation latent virus (CLV) are the most important viruses affecting carnation crops. All except CERV are RNA viruses. Viral RNA or DNA accumulation on root, stem, leaf, sepal, petal, stamen, pistil and ovary tissues of infected carnation or plants was analysed by non-isotopic molecular hybridisation.