Massive immuno multiresidue screening of water pollutants.

An immuno multiresidue screening assay in microarray format for the determination of complex chemical mixtures at the microgram per liter level, using antibody-functionalized gold nanoparticles, is presented. The analytical method relies on the use of a cocktail of nanogold-labeled specific antibodies, acting as recognition and detection species. The concept of multireside screening is proved by developing a multiplex assay on a compact disk support for the determination of 2-(2,4,5-trichlorophenoxy)propionic acid, 3-phenoxybenozic acid, 4-nitrophenol, alachlor, atrazine, azoxystrobin, chlorpyrifos, diazinon, diuron, endosulfan, fenthion, forchlorfenuron, imidacloprid, malathion, pentachlorophenol, pyraclostrobin, sulfasalazine, and triclosan, achieving detection limits of 0.

Selective enzyme-linked immunosorbent assay for triclosan. Application to wastewater treatment plant effluents.

A sensitive and highly selective enzyme-linked immunosorbent assay was developed for triclosan, one of the most common bactericides in personal care products, which is also considered as an emerging contaminant, given its presence in the effluents of sewage and wastewater treatment plants. From synthesized haptens, polyclonal rabbit antibodies against triclosan were raised. The best ELISA immunoassay was based on an antibody-coated format, yielding a detection limit of 0.