Identification and Characterization of Mediators of Fluconazole Tolerance in .

is an important human pathogen and a major concern in intensive care units around the world. infections are associated with a high mortality despite the use of antifungal treatments. One of the causes of therapeutic failures is the acquisition of antifungal resistance by mutations in the genome.

Comparative Genomics for the Elucidation of Multidrug Resistance in Candida lusitaniae.

Multidrug resistance (MDR) has emerged in hospitals due to the use of several agents administered in combination or sequentially to the same individual. We reported earlier MDR in during therapy with amphotericin B (AmB), azoles, and candins. Here, we used comparative genomic approaches between the initial susceptible isolate and 4 other isolates with different MDR profiles.

Genetic screens reveal novel major and minor players in magnesium homeostasis of Staphylococcus aureus.

Magnesium is one of the most abundant metal ions in living cells. Very specific and devoted transporters have evolved for transporting Mg2+ ions across the membrane and maintain magnesium homeostasis. Using genetic screens, we were able to identify the main players in magnesium homeostasis in the opportunistic pathogen Staphylococcus aureus.