Publications by authors named "Emilie Seljeseth"
Article Synopsis
- The HAP1 human cell line, which is near-haploid, is popular for CRISPR/Cas9 gene editing because it simplifies the editing process by only needing one allele modified.
- Researchers found that HAP1 cells can become diploid over time, which may impact experiments, necessitating control over the cell line's ploidy status.
- The article presents optimized protocols for determining and controlling the ploidy of HAP1 cells using flow cytometry and size-based cell sorting to ensure reliable experimental outcomes.
