Key Role of Staphylococcal Fibronectin-Binding Proteins During the Initial Stage of Keratitis in Humans.

Objectives: is one of the main causes of bacterial keratitis in humans. This study was aimed at investigating the mechanisms of adhesion to the human corneal epithelium involved during the initial stage of infectious keratitis.

Methods: Human corneas stored in a specific active storage machine that restores a normal pluristratified epithelium were used to assess adhesion level to intact and injured tissues using immunostaining.

New Freeware for Image Analysis of Lissamine Green Conjunctival Staining.

Purpose: Lissamine green (LG) is often used in addition to fluorescein to assess the severity of conjunctival damage in dry eye syndrome, which is graded manually. Our purpose was to describe an algorithm designed for image analysis of LG conjunctival staining.

Methods: Twenty pictures of patients suffering from dry eye with visible LG conjunctival staining were selected.

One threat, different answers: the impact of COVID-19 pandemic on cornea donation and donor selection across Europe.

Objectives: To assess to which extent the COVID-19 pandemic affected corneal transplantation by virtue of donor selection algorithms in different European countries.

Design: Survey.

Setting: 110 eye banks in 26 European countries.

Tolerance to Light of Patients Suffering From Infectious Keratitis.

Purpose: With very photophobic patients, the advantages of red or near infrared light to develop new ophthalmology imaging devices seem obvious: no or little glare, possibility of long signal integration, no phototoxicity, and lesser autofluorescence of ocular tissues. Nevertheless, in this range, the shortest possible wavelength facilitates signal detection. The aim of this study was, thus, to determine the maximal irradiance tolerated with 6 wavelengths: 2 red, 2 far red, and 1 near infrared lights to determine the shortest wavelength well tolerated by patients, in comparison with the standard cobalt blue light of ophthalmology slitlamp.

Ex vivo model of herpes simplex virus type I dendritic and geographic keratitis using a corneal active storage machine.

Background: Herpetic keratitis (HK) models using whole human corneas are essential for studying virus-host relationships, because of high species specificity and the role of interactions between corneal cell populations that cell culture cannot reproduce. Nevertheless, the two current corneal storage methods (hypothermia and organ culture (OC)) do not preserve corneas in good physiological condition, as they are characterized by epithelial abrasion, stromal oedema, and excessive endothelial mortality.

Methods: To rehabilitate human corneas intended for scientific use, we used an active storage machine (ASM) that restores two physiological parameters that are essential for corneal homeostasis: intraocular pressure and storage medium renewal (21mmHg and 2.

Phacoemulsification And Zonular Weakness: Contribution Of The Capsular Tension Ring With A Thread.

Purpose: Cataract surgery in patients suffering from zonular weakness can be difficult and lead to severe complications. The insertion of capsular tension rings to stabilize the capsular bag along all steps of the phacoemulsification is an option, although can be difficult to remove intra- or post-operatively. A capsular tension ring with a thread is a safe alternative, which allows its easy removal at the end of the surgery.

Micro-instillation of fluorescein with an inoculation loop for ocular surface staining in dry eye syndrome.

Purpose: To describe and validate the micro-instillation of fluorescein on the ocular surface by a disposable calibrated inoculation loop to improve corneal and conjunctival staining quality.

Methods: Accuracy and precision of the volume of 0.5% sodium fluorescein collected by a single use 1 μl-calibrated inoculation loop were measured using a precision balance.

Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts.

Purpose: Engineered corneal endothelial grafts able to provide numerous functional endothelial cells for the restoration of corneal transparency would be a worthwhile way of replacing donor tissue, which is extremely scarce. The grafts are simply constructed: a biocompatible thin and transparent carrier colonized by a monolayer of cultured endothelial cells (ECs). Here we describe a process able to obtain appropriate carriers by recycling human corneas unsuitable for graft in their original state, but liable to provide multiple thin lamellae when cut with a femtosecond laser as used in refractive surgery.

Size of the Lesions of Superficial Punctate Keratitis in Dry Eye Syndrome Observed With a Slit Lamp.

Purpose: To evaluate the size distribution of epithelial lesions of superficial punctate keratitis (SPK) in dry eye after staining of the ocular surface by sodium fluorescein.

Methods: Fluorescein was instilled in 10 patients with dry eye graded using the Oxford Scheme. Pictures were taken using a standard Topcon slit lamp with cobalt blue light, without barrier filter.