Nanobody-based sensors reveal a high proportion of mGlu heterodimers in the brain.

Membrane proteins, including ion channels, receptors and transporters, are often composed of multiple subunits and can form large complexes. Their specific composition in native tissues is difficult to determine and remains largely unknown. In this study, we developed a method for determining the subunit composition of endogenous cell surface protein complexes from isolated native tissues.

A novel method to detect hPG (human circulating progastrin) in the blood.

hPG (human circulating progastrin) is produced and released by cancer cells. We recently reported that hPG is detected in the blood of patients with cancers from different origins, suggesting its potential utility for cancer detection. To accurately measure hPG in the blood of patients, we developed the DxPG test, a sandwich Enzyme-Linked Immunosorbent Assay (ELISA).

An Innovative Multiplexed and Flexible Molecular Approach for the Differential Detection of Arboviruses.

Nucleic acid testing during the preseroconversion viremic phase is required to differentially diagnose arboviral infections. The continuing emergence of arboviruses, such as Zika virus (ZIKV), dengue virus (DENV), and chikungunya virus (CHIKV), necessitates the development of a flexible diagnostic approach. Similar clinical signs and the priority to protect pregnant women from ZIKV infection indicate that the differential diagnosis of arboviruses is essential for effective patient management, clinical care, and epidemiologic surveillance.

Allosteric nanobodies uncover a role of hippocampal mGlu2 receptor homodimers in contextual fear consolidation.

Antibodies have enormous therapeutic and biotechnology potential. G protein-coupled receptors (GPCRs), the main targets in drug development, are of major interest in antibody development programs. Metabotropic glutamate receptors are dimeric GPCRs that can control synaptic activity in a multitude of ways.

Early Sitting in Ischemic Stroke Patients (SEVEL): A Randomized Controlled Trial.

Background: Extended immobility has been associated with medical complications during hospitalization. However no clear recommendations are available for mobilization of ischemic stroke patients.

Objective: As early mobilization has been shown to be feasible and safe, we tested the hypothesis that early sitting could be beneficial to stroke patient outcome.

MESOSCOPIC MODELING OF STOCHASTIC REACTION-DIFFUSION KINETICS IN THE SUBDIFFUSIVE REGIME.

Subdiffusion has been proposed as an explanation of various kinetic phenomena inside living cells. In order to fascilitate large-scale computational studies of subdiffusive chemical processes, we extend a recently suggested mesoscopic model of subdiffusion into an accurate and consistent reaction-subdiffusion computational framework. Two different possible models of chemical reaction are revealed and some basic dynamic properties are derived.

Design and validation of a homogeneous time-resolved fluorescence cell-based assay targeting the ligand-gated ion channel 5-HT3A.

Ligand-gated ion channels (LGICs) are considered as attractive protein targets in the search for new therapeutic agents. Nowadays, this strategy involves the capability to screen large chemical libraries. We present a new Tag-lite ligand binding assay targeting LGICs on living cells.

A time-domain numerical modeling of two-dimensional wave propagation in porous media with frequency-dependent dynamic permeability.

An explicit finite-difference scheme is presented for solving the two-dimensional Biot equations of poroelasticity across the full range of frequencies. The key difficulty is to discretize the Johnson-Koplik-Dashen (JKD) model which describes the viscous dissipations in the pores. Indeed, the time-domain version of Biot-JKD model involves order 1/2 fractional derivatives which amount to a time convolution product.