Formation of multiple-oocyte follicles in culture.

Basement membranes are found in every organ of the body. They provide structure and a selective filter for molecules. The ovary is no different with the follicular basal lamina (FBL) separating the granulosa and theca cells, facilitating regulation of the changing follicular environment providing appropriate conditions for the developing oocyte.

Oocyte-derived Smad4 is not required for development of the oocyte or the preimplantation embryo.

Unlabelled: The generation of a competent egg requires complex molecular interactions between the oocyte and the ovary, and transforming growth factor β (TGF-β) is a major signaling pathway. Smad4 is a central regulator of the TGF-β signaling pathway as it mediates gene expression triggered by activation of TGF-β receptors. Deletion of Smad4 in granulosa cells disrupts follicle development; however, the role of Smad4 in the oocyte has not been confirmed.