In the present work it was shown that biosynthesis of silver sulfide nanoparticles from silver nitrate and sodium thiosulfate solutions of millimolar concentration occurs efficiently by living Shewanella oneidensis MR-1 cells, as well as by ultrasonically-disrupted cells and by the membrane fraction of the cells. The size of nanoparticles synthesized in the presence of living cells was 7.8 ± 1.
View Article and Find Full Text PDFIt was stated that spaceflight factors (SFF) affect the chromosomal DNA interchange during Streptomyces crossing. Cross polarity and primary input of a parent chromosome fragment in recombinant generation imply a more lasting cells contact in microgravity and a broader horizontal transport of genetic material. SFF had no effect on recombination frequency and mutation in a model of parental auxotrophic markers reversion to prototrophism.
View Article and Find Full Text PDFThe aim of study was to investigate static balance control in wrestlers (n = 31) and effects of fatigue on postural regulation in two position: bipedal stance and squat position with open eyes prior to and 2 min after bicycle PWC(170) test. A force platform ("Ritm". Russia) was used to determine static balance control.
View Article and Find Full Text PDFPurpose of the work was designing and prototyping of microbial fuel cells (MFC) and comparative evaluation of the electrogenic activity of wastewater autochthonous microorganisms as well as bacterial monocultures. Objects were model electrogenic strain Shewanella oneidensis MR-1, and an Ochrobactrum sp. strain isolated from the active anode biofilm of MFC composed as an electricity generating system.
View Article and Find Full Text PDFThe goal of study was to investigate the regulation of balance in athletes with different sports qualifications: masters of sport (MS, n=18) and candidates for master of sports (CMS, n=13). Balance examined by means of stabilographic complex ("Rhythm". Russia) in the static tests: in simple bipedal stance (BS) and squat positions (SQ), as well as in the dynamic tests: "Involute", evaluating the tracking movement and "Step input", assessing the reaction of the whole body on the visual-motor task.
View Article and Find Full Text PDFSince the first description of sympatric sibling species and allopatric chromosomal forms of the common vole (Microtus arvalis group) in 1969, the search of their range boundaries has been Continually going on up to the present time. Based on the cytogenetic material presented in the present study, the geographical distribution of two karyoforms (2n = 46: NF = 84 and NF = 72) was examined in 20 geographic localities of European Russia (within the interval between the longitude 34 degrees-56 degrees East and 51 degrees-61 degrees). The places of findings of two karyoforms, "arvalis" and "obscurus", in Russia have been supplemented and were shown to be significantly closer; whereas the well-known distribution boundaries for the "obscurus" form were specified in the northern and southern parts of Russia.
View Article and Find Full Text PDFZh Evol Biokhim Fiziol
June 2009
In this review, causes of tremendous differences in respiration and oxidative metabolism between the homoiothermal and poikilothermal vertebrates are discussed. Tissues of the latter contain not lower, and sometimes even higher amount of mitochondria with the powerful potential of oxidative enzymes, as compared with the homoiothermal vertebrates; this potential is realized only by 3-4% because of a low rate of oxygen transport due to peculiarities of the cardiovascular system of the lower vertebrates. Limitation of ATP synthesis is responsible for a specific structure of plasma membranes with the essentially lower amount of channels for the passive ion leakage as compared with the mammalian plasma membranes.
View Article and Find Full Text PDFThe work dealt with study of mitochondria in reversible metabolic suppression of hepatocytes of the river lamprey Lampetra fluviatilis in the course of prespawning starvation and of liver mitochondria of the common frog Rana temporaria during hibernation and activity. In winter the metabolic depression of lamprey hepatocytes, unlike that of frog hepatocytes, has been found to be due to deactivation of complex I of the electron transport mitochondrial chain, a low rate of NAD-dependent substrate oxidation, a low content of adenine nucleotide content, and a high degree of mitochondrial membrane permeability to H+ and other monovalent ions (KCl-, K+). The mitochondrial membrane permeability decreases in the presence of ethyleneglycoldiamineethyltetraacetic acid (EGTA), cyclosporine A (CsA), adenosine-5'-diphosphate (ADP), and Mg+.
View Article and Find Full Text PDFMethod of surgical chemotherapy on organism automedia during surgical procedures for breast and colon malignant tumors has been developed. The method consists in intravenous autohemochemotherapy and local autoplasmochemotherapy (into residuary mammary tissue or regional arterial mesenteric vessel).
View Article and Find Full Text PDFNow there is no generally accepted practice of antibacterial therapy of acute sinusitis in outpatient clinics of Russia. Choice of antibacterial drugs is often made without consideration of the most probable causative agents of the infection. Out-of-date antibiotics used in many cases do not satisfy modern requirements.
View Article and Find Full Text PDFRoss Fiziol Zh Im I M Sechenova
April 2004
Free amino acids in the blood plasma of lamprey (Lampetra fluviatilis) and frog (Rana temporaria) have been determined quantitatively for the periods of deprivation of the exogenous feed. The lamprey's total amino acid pool increased by 74% from November to April and reached the lower limit known for the mammals. The amount of free amino acids in frogs decreased by 40% in the spring as compared with the autumn values.
View Article and Find Full Text PDFThe programme was aimed at audit of the parameters of antibacterial therapy for acute otitis media in adult outpatients in 8 cities of Russia (Smolensk, Volgograd, Ekaterinburg, Yaroslavl, Nizhny Novgorod, Tyumen, Ryazan, Vladivostok). The information sources were the case records. The data from the records were structurally fixed in specially designed individual registration charts for further computer processing.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
February 2004
The main penetration route of cryptosporidia oocysts (at the infective stage) from animal carriers in the natural ecosystems of the forest zone into the environment of human habitat has been newly established. Oocysts excreted with feces are concentrated in the surface layer of the soil, then washed into water reservoirs. The viability of oocyts from the soil and the silt of river sand banks and shallows has been experimentally shown.
View Article and Find Full Text PDFA new method of plasmid DNA transfer from the donor strain Escherichia coli S17-1 to the erythomycin-producing strain Saccharopolyspora erythraea and avermectin-producing strain Streptomyces avermitilis via intergeneric conjugation was proposed. The optimal parameters of the method were chosen for increasing the efficiency of crosses and ensuring easily reproducible results. The behavior of the multicopy plasmid pPM803 and the integration vector pTO1 along with a number of new plasmids specially created by us, was examined in these strains.
View Article and Find Full Text PDFBased on the results of examination of 30 patients with hormonally active adrenal tumors, the authors consider the MRI sympatomatology of their different types and the potentialities of the technique in the complex radiation diagnosis of this pathology. The authors present their assessments of the relative intensity of a signal and the structure of each type of hormone-producing tumors of the glands by using different MRI pulse sequences that may be useful in establishing a presumptive morphological diagnosis. They identify MRI sequences that are of the greatest informative value for the diagnosis of each type of hormonally active adrenal tumors.
View Article and Find Full Text PDFBased on the findings of 54 magnetic resonance studies, the authors used 19 of them authors to study the types of normalcy. A role of the new noninvasive technique magnetic resonance cholangiopancreatography (MRCPG) in the diagnosis of hepatopancreatoduodenal diseases is assessed. The potentialities of MRCPG in the detection of most common diseases and malformations of the biliary system are demonstrated.
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