[Effect of antihypoxant infusion on microbial endotoxin circulation in obstructive jaundice patients].

The dynamics of microbial endotoxemia in group of 149 patients with cholelithiasis complicated by obstructive jaundice has been studied as dependent on the perioperative tactics of infusion therapy. The perioperative period in obstructive jaundice patients is complicated by a significant increase in lipopolysaccharidemia caused by translocation mechanisms and disorders of the liver detoxification function. In Group 1, 47 patients received infusion therapy including Ringer's solution and 10% glucose solution at a 1:1 ratio.

[Tactics of preoperative infusion therapy in obturation jaundice].

The influence of the infusion therapy content on a hepatic function of bile outflow (HFBO), a bilirubin content and the enzymes activity in obturation jaundice in 139 patients was studied up. In 54 patients (the first group) a Ringer solution and 10% solution of glucose in 1:1 ratio were applied; in 37 patients (the second group) - a sterofundin-G-5 solution; in 48 patients (the third group) - remaxol in a dose of 800 ml/day. Application of sterofundin-G-5 and remaxol as a component of infusion therapy have promoted a bile outflow intensity enhancement in early postoperative period in comparison with such while Ringer solution and 10% solution of glucose application.

[CYP2C9 and VKORC1 gene polymorphism and acenocoumarol anticoagulant activity in Russian patients at high risk of thromboembolic complications].

The study included 25 patients at high risk of thromboembolic complications. All of them were treated with acenocoumarol for 6 months under control of the frequency of hemorrhage and episodes of severe hypocoagulation (a more than 3-fold rise in INR). All the patients underwent CYP2C9 and VKORC1 genotyping.

[Infusion correction of bile outflow and endotoxicosis in patients with mechanical jaundice].

The investigations were performed in 139 patients divided into 3 groups and who were given similar volume of infusion therapy (1600 ml/day). In the first group consisting of 54 patients the solution of infusion therapy included the Ringer solution and 10% of glucose solution in the ratio 1:1. In the second group (37 patients) the infusion therapy was performed with a solution of sterofundin-G-5.

[Clinical and pathogenetic significance of antibodies to antioxidative enzymes in patients with Raynaud's syndrome].

Patients presenting with systemic scleroderma were found to have antibodies to antioxidative enzymes the levels of which increased with activity of the disease. Taking into account the important role of immune disorders in the development of systemic sclerosis, it can be conjectured that antienzyme antibodies may cause dysregulation of enzymatic systems. Serum antibodies were detected by the original modification of indirect enzyme immunoassay using immobilized antigenic forms of enzymes.

[Interrelationship between antibodies to enzymes of antioxidant system and cardiac involvement in patients with systemic scleroderma].

In blood of patients with systemic scleroderma we detected antibodies to the antioxidant system enzymes. Level of specific immunoglobulins rose with increase of the disease activity. Antioxidant system of the body is represented by a number of enzymes called to negate pathogenic effect of active forms of oxygen.

[Effect of corticotropin-releasing factor on anoxia-induced changes in evoked potentials in cultured tissue of the rat olfactory cortex].

The experiments were performed on incubated slices of the rat rostal olfactory cortex. The slices were treated with various concentrations (10(-7) to 10(-9)) of corticotropin-releasing factor before (40 min), during, and after a 10-minute anoxic episode. The parallel control experiments were carried out without CRF.

[Effect of various concentrations of corticoliberin on long-term potentiation in slides of the rat olfactory cortex].

Perfusion of the rat olfactory cortex slices with 1 mcM corticotropin-releasing factor (CRF) increased the rate of initiation of the posttetanic potentiation. The latter had a shorter supporting phase than in the control. With the perfusion, with 0.

[45Ca metabolism in slices of rat cerebral cortex during long-term potentiation].

Ca45 kinetics was studied in 5, 15 and 30 min after potentiation. In the induction phase (1-5 min), the potentiation decreased the fraction of intracellular bound Ca. The 15-25 min potentiation the intra- and extracellular Ca levels was equal to the control ones.

[The molecular-cellular mechanisms of long-term potentiation].

The review of literature data is presented on the problem molecular and cellular mechanisms of learning which is intensely studied for last decade using the long-term potentiation (LTP) model in the brain slices in vitro. The main attention is paid for molecular mechanisms of LTP induction and supporting phases in hippocampal structures on the pre- and postsynaptic levels especially for the involvement of transmitters and retrograde messengers in these processes. The role of protein kinase-dependent proteins phosphorylation and of peptides synthesis expression in the LTP development is discussed.

[Correlates of membrane metabolism and long-term potentiation in slices of rat cerebral cortex].

A group of interrelated parameters of the membrane metabolism was studied during stimulation of the lateral olfactory tract in the rat brain slices of olfactory cortex. All the parameters were studied in respect to electrical activity of the slices in three temporal points after the tetanisation: 3-5, 15, and 30 min. Regular phasic alterations of the metabolism parameters occurred in most of the cases.

[The functional plasticity of the cells of the paraventricular nucleus in surviving slices of the hypothalamus].

In surviving cuts of the hypothalamus, stimulation of the supraoptic nucleus induced focal potentials in the paraventricular nucleus. A repeated stimulation was followed by a reduction of the descending of the focal potentials. Tetanization of the supraoptic nucleus induced an additional wave at the descending phase of focal potentials preserving for 15 min.

[The effect of picrotoxin on phospholipid methylation in the olfactory cortex of the rat brain].

Within 15 min after addition of picrotoxin to incubation medium, a reduction of the phosphatidylethanolamine methylation occurred. The reduction seems to be related to opening of ion channels since during this period of the picrotoxin action the maximal membrane depolarisation takes place.

[Effect of hydrocortisone on phosphoinositide metabolism in rat cerebral cortex slices].

Hydrocortisone administered in a dose of 1 and 5 mg per 100 g of mass has increased the rate of 32P turnover in di- and triphosphatidyl inositides up to 170-230% and has no influence on the content of these phospholipids.

[The effect of the tripeptide melanostatin on phospholipid metabolism in the olfactory cortex of rats].

The incubation of the brain slices with melanostatine for 15 min increased the contents and reduced the metabolism intensity of phosphatidylcholine and phosphatidyletanolamine in the rat olfactory cortex. The sphingomyelin metabolism intensity was increased. These shifts in the metabolism of membraneous components may cause considerable modifications in the membranes properties and can be a material basis for long-term changes in the unit activity.

[The effect of hydrocortisone on posttetanic potentiation in rat cerebral cortex slices and the molecular mechanisms of this effect].

The effect of hydrocortisone on posttetanic potentiation, Ca exchange, metabolism of phosphoinositides and phospholipids methylation, was studied. Hydrocortisone (10(-4) M) facilitated and prolonged the posttetanic potentiation. Incubation of slices in hydrocortisone for 90 sec elevated the incorporation of 32P-orthophosphate in polyphosphoinositide.

[Posttetanic and frequency potentiation in slices of the olfactory cortex from the rat brain].

A 15-sec tetanization of lateral olfactory tract at the frequency of 30/sec produced a short-term (20 min) posttetanic potentiation of the field potentials (FPs) in the rat olfactory cortex slices. Rhythmical stimulation of lateral olfactory tract (6 trains of 10 pulses each at the frequency of 10/sec, with 4-5 sec intertrain interval) evoked a progressive increase in the amplitude of postsynaptic components of the FPs (EPSP and IPSP) but not in amplitude of presynaptic component--the compound AP of lateral olfactory tract. The findings suggest the development of the 2-form functional plasticity: posttetanic and frequency potentiation in pyriform cortex.

[The neurotropic effects of hydrocortisone in surviving slices of the rat olfactory cortex].

The effects of hydrocortisone on short-term habituation, post-tetanic potentiation, fluctuations of the amplitude of focal potentials (FPs) and slow after-hyperpolarization were studied in the olfactory cortex slice preparations. Hydrocortisone (10(-5)-10(-4) M) inhibited the habituation but facilitated and prolonged the post-tetanic potentiation of the FPs. The hormone increased the fluctuations of the FPs and exerted no effect on slow after-hyperpolarization evoked by a brief orthodromic stimulation.

[Effect of hydrocortisone on synaptic transmission in surviving slices of the olfactory cortex from the rat brain].

A study was made of the effect of bath-applied hydrocortisone (10(-4) M) on excitatory transmission of the lateral olfactory tract (LOT) - superficial pyramidal cell synapse of the rat olfactory cortex slice. Population EPSP and IPSP were depressed for 6-12 min., then they were increased (60 min.

[Effect of the tripeptide melanostatin on the electrical activity of surviving slices of the olfactory cortex in the rat brain].

The effects of melanostatin (MST 10(-5) M) on transmission in the lateral olfactory tract--superficial pyramidal neurons synapses, were investigated in slices of the rat olfactory cortex. Within 3-6 min after MST addition, the EPSP amplitudes were depressed and then increased (within 6-9 to 30 min); spikes and IPSPs appeared during the latter period. The recovery of the FPs to control values at washing was slow (40-60 min).

[Effect of hydrocortisone on the level and metabolism of phospholipids in the rat cerebral cortex].

Hydrocortisone was studied for its effect in vivo and in vitro on the phospholipids content and metabolism in the rat brain slices under conditions of their incubation in the medium with [2-14C]acetate. The seven-day administration of the preparation increases the specific radioactivity of sphingomyelin 2 times and that of phosphatidyl serine 2.3 times.

[Metabolism of 22Na in the rat corpus callosum].

The kinetic parameters of 22Na+ exchange were measured with improved compartmental analysis in slices of rat corpus callosum. Three distinct Na compartments were found: 45, 38 and 15% of the total Na+, having T1/2 for efflux 7.3; 43.