[Connection of perforating and intramuscular veins of the crus in varicosity].

Aim: This study was undertaken to investigate the clinical anatomy of indirect perforating veins and their connection to the intramuscular venous collector of the crus by means of MSCT phlebography.

Patients And Methods: From 2015 till now, MSCT phlebography was used to examine a total of 400 patients with chronic diseases of lower limb veins. According to the CEAP classification, clinical class C0-C1 was present in 108 (27%) subjects, C2-C3 - in 173 (43.

[A device for endoluminal thermal obliteration of varicose veins].

Amongst the techniques of endoluminal thermal obliteration of varicose veins, the most commonly employed treatment modalities are endovasal radiofrequency ablation and endovasal laser obliteration, both using a generating source and a special device for carrying out thermal obliteration. It has been noted that each of the methods of thermal obliteration has a number of disadvantages, irrespective of using the energy of magnetic oscillations in a radiofrequency range or laser radiation, including high cost of expendable materials and equipment. The device we worked out for endoluminal thermal obliteration of varicose veins was based on the principle of high efficacy and safety, with a significant decrease in the cost of carrying out the manipulation.

[A new-type device for endovasal laser obliteration of varicose veins].

The purpose of the study was to work out a Russian-made, simple-to-manufacture, safe, inexpensive and efficient device for performing endovasal laser obliteration (EVLO) of varicose veins, with the underlying principle of endoluminal distribution of the total laser energy into several flows. The development of this device was based on the principle of dividing the total laser radiation into two energy flows. One flow has a shape of circularly formed, continuous field of laser radiation of specified width within the angular range from 50 to 90°, which ensures the main energetic contribution to thermal impact on the vascular wall.

[Retrospective analysis of results of using cyanoacrylate composition for varicose vein ablation].

The method of cyanoacrylate ablation of varicose veins is currently considered as a possible alternative to traditional methods of thermal obliteration. One of significant advantages of this method is no need for performing tumescent anaesthesia. Over the period from 1995 to 2002, cyanoacrylate obliteration of varicose veins was carried out in a total of 96 patients, with clinical assessment of the complication rate and efficacy at various time intervals, with the maximum follow-up period amounting to 3 years.

[Thermal stability of lactate dehydrogenase in the complex with the anion polyelectrolyte poly(styrene sulfonate)].

The temperature stability of the cytoplasmic enzyme of the glycolysis of lactate dehydrogenase from a pig muscle (isoenzyme M4) in a complex with the anion polyelectrolyte poly(styrenesulfonate) has been investigated by the methods of adiabatic differential scanning microcalorimetry, the own protein fluorescence, and circular dichroism. Calorimetric investigations of complex of lactate dehydrogenase with poly(styrenesulphonate) in 50 mM phosphate buffer at pH 7.0 have shown that the temperature of the transition and enthalpy of lactate dehydrogenase thermal denaturation sharply decreases with growing weight ratio poly(styrenesulphonate)/lactate dehydrogenase, though at 20 degrees C the enzyme activity of lactate dehydrogenase remains unchanged for several hours irrespective of the addition of poly(styrenesulphonate).

[The principles of analysis on multicomponent fluorescence spectra of proteins. Temperature and pH-induced transitions in tetrameric melittin].

Structural transitions in the tetrameric melittin from bee venom in 2 M KCl induced by variations of pH (from 0.7 to 12.0) and temperature (from 2 to 95 degrees C) have been studied.

[Sarcomeric proteins of the titin family form amyloids].

It was shown for the first time that skeletal muscle sarcomeric proteins of the titin family (X-, C- and H-proteins) are able to form in vitro amyloid aggregates of different types: granular aggregates, protofibrils, helically twisted ribbons, linear fibrils, and bundles of linear fibrils. Their amyloid nature was confirmed by electron, polarization, and fluorescence microscopy and by spectral methods. As opposed to other amyloidogenic proteins, X-, C-, and H-proteins easily form amyloids under mild conditions close to physiological ones (pH, ionic strength, temperature).

[The component analysis of tryptophan fluorescence spectra of melittin during its oligomerization].

The oligomerization of melittin with increasing ionic strength and protein concentration was investigated using the methods of decomposition of its tryptophan fluorescence spectra into "elementary" log-normal components. At high ionic strength (up to 2 M KCl), the emission spectra of tetrameric melittin are well described as the sum of two log-normal components, suggesting the presence of tryptophan residues in two sorts of environment with greatly differing polarity. Measurements of fluorescence spectra by iodide showed that these two spectral components possess different Stern-Volmer constants, that is, the tryptophans emitting them have different solvent accessibility, which does not correlate with the crystallographic structure of tetrameric melittin.

[Diagnosis of coronary atherosclerosis using echocardiography with bicycle ergometry exercise].

Aim: To determine sensitivity and specificity of stress echocardiography (SEC) in diagnosis of stenosing atherosclerosis of coronary arteries (CA), especially in patients recovered from Q-myocardial infarction.

Material And Methods: The above sensitivity and specificity were studied in 75 patients (70 males and 5 females; mean age 53.7 +/- 7 years) with coronary atherosclerosis (CS).

[Prognostication of the course of stable ischemic heart disease by exercise stress echocardiography].

We analyzed data from 549 patients (450 with high and 99 -- low pretest probability of ischemic events). Duration of observation was 12-42 (mean 20.1+/-11.

Results of vascular reconstructions for a associated lesion of the brachiocephalic arteries and paroxysmal disorders of cardiac rhythm.

Annually, about 400,000 new patients with strokes are recorded in Russia. Of these, 80% of patients may develop ischemic brain infarctions. In approximately 30-40% of cases, ischemic lesion of the brain eventuates in lethal outcome; the same number of patients remain heavily disabled and only 15-20% of patients return to the previous level of work fitness.

[Possibilities of stress-echocardiography in diagnostics of pathology of mitral valve. Part II].

The paper deals with different aspects of application of stress-echocardiography for assessment of mitral valve disease (acquired stenosis or insufficiency, mitral regurgitation due to ischemic heart disease and mitral valve prolapse). Continuity equation is preferable to pressure half-time and resistance for estimation of hemodynamic significance of mitral stenosis. In some patients studies at rest reveal only slight mitral regurgitation while during stress it becomes hemodynamically significant and associated with pronounced elevation of pulmonary artery pressure.

[Analysis of log-normal components of fluorescence spectra of prodan and acrylodan bound to proteins].

Steady-state fluorescence spectra of prodan and acrylodan covalently bound to cystein residue of Lys-Cys-Phe tripeptide in solvents of different polarity were analyzed. It was shown that the shape of spectral bands is well described by a log-normal function. Linear relations between three shape-determining parameters of the log-normal function (namely, the positions of spectral maximum and two half-maximum amplitudes) were revealed and evaluated for both fluorophores.

[Structural modification of environment by microorganisms: formation of Liesegang rings around Dictyostelium discoideum population].

The results of the experimental study of the environment modification--the emergence of Liesegang rings around a Dictyostelium discoideum population are presented. The formation of Liesegang rings induced by D. discoideum cells is observed on addition of glucose into the semi-solid nutrient medium (agar concentration 0.

[The means for improving therapeutic care in the Navy].

The army elements of the medical service, as the results of the checks have shown, have more than 80% of all defects of the therapeutic care providing at the fleet. Specific character of work of the ships doctors consists in long duration of the services, during which the doctor should independently decides any problems of providing medical care. Growing threat of large-scale radiating and toxicological disasters, presence at Naval Fleet of the seamen from the special contingent structure dictates necessity of the specialized medical care organization to appropriate categories of patients and injured.

[The role of temperature as a factor of structure and functional fit of a "prey" virus based on the example of phage T4].

By means of high-precision acoustic measurements and by the methods of fluorescent and electron microscopy investigations were performed of thermoinduced conformational changes in T4 bacteriophage and its thermolabile mutants altered in baseplate proteins (gene products "7", "8", "10"). A relationship was found between the conformational changes in T4 bacteriophage structure in the temperature range of 33-45 degrees C and the efficiency of bacteriophage adsorption and changes in the orientation of long tail fibers. Possibility of heat regulation of "recognition" of "host" cells by bacterial viruses is suggested.