The Effect of Three Different Biomaterials on Proliferation and Viability of Human Dental Pulp Stem Cells (In-vitro Study).

Background: Biomaterial cytotoxicity on dental stem cells plays a critical role in managing the regeneration of dental tissue.

Aim: The aim of the present study was to evaluate the effect of Nano-hydroxy apatite (NHA), Mineral trioxide aggregate (MTA), and Calcium-enriched mixture (CEM) on the proliferation, and viability of human dental pulp stem cells (hDPSCs) isolated from third molar teeth.

Methods: Cultured DPSCs were characterized and the tested biomaterials were shaped into cylinders then inserted directly on the DPSCs.

Clinical and molecular characterization of seven Egyptian families with autosomal recessive robinow syndrome: Identification of four novel ROR2 gene mutations.

Robinow syndrome (RS) is a rare genetic disorder characterized by limb shortening, genital hypoplasia, and craniofacial/orodental abnormalities. The syndrome follows both autosomal dominant and recessive patterns of inheritance with similar phenotypic presentation and overlapping features. Autosomal recessive Robinow syndrome (ARRS) is caused by mutations in the ROR2 gene.

Stem cells from human exfoliated deciduous teeth--isolation, long term cultivation and phenotypical analysis.

Aims: Our aims were to isolate stem cells from human exfoliated deciduous teeth (SHED), to cultivate them in vitro and to investigate their basic biological properties, phenotype and to compare our findings with dental pulp stem cells (DPSC) isolated from permanent teeth.

Methods: Dental pulp was gently evacuated from exfoliated teeth. After enzymatic dissociation of dental pulp, SHED were cultivated in modified cultivation media for mesenchymal adult progenitor cells containing 2% FCS and supplemented with growth factors and insulin, transferrin, sodium (ITS) supplement.