Effect of silver nanoparticles on tropane alkaloid production of transgenic hairy root cultures of Hyoscyamus muticus L. and their antimicrobial activity.

The utilization of nanotechnology and biotechnology for enhancing the synthesis of plant bioactive chemicals is becoming increasingly common. The hairy root culture technique can be used to increase secondary metabolites such as tropane alkaloids. Agrobacterium was used to induce hairy roots from various explants of Hyoscyamus muticus.

De novo expression and antibacterial potential of four lactoferricin peptides in cell-free protein synthesis system.

For the first time, we produced four lactoferricin (LFcin) peptides by a cell-free () method. These short antimicrobial peptides were expressed in an cell-free protein synthesis (CFPS) system and the bioactivity of the produced peptides was demonstrated. Additionally, we designed a novel synthetic consensus peptide (ConLFcin).

Purification and biotechnological applications of L-asparaginase from newly isolated OHEM18 as antitumor and antioxidant agent.

In the present study, a new bacterial strain, OHEM18 was significantly found to produce extracellular L-asparaginase. L-asparaginase was purified using ammonium sulfate precipitation and QFF column to 3.84-fold with specific activity of 215.

Production, optimization, purification, characterization, and anti-cancer application of extracellular L-glutaminase produced from the marine bacterial isolate.

L-glutaminase from bacterial sources has been proven to be effective and economical agents in cancer therapy, food industry and high-value chemicals like threonine. In the present study, a newly isolated bacterial strain was potentially producing extracellular L-glutaminase, it identified as OHEM11 (MK389501) using the gene. L-glutaminase production optimized and the optimum factors for production under submerged fermentation were at pH 6.

Synthesis and investigations on tellurium myconanoparticles.

Tellurium has attracted the attention of many researchers and manufacturers due to its unique properties. Through the current work, six fungal isolates have been screened for their ability to reduce potassium tellurite (KTeO) into elemental tellurium nanoparticles (TeNPs). The most promising fungal isolate was identified as and given the accession number (KY766958) based on molecular basis and has been used for biogenic (enzymatic) production of TeNPs.