Cytotoxicity of Methacrylate Dental Resins to Human Gingival Fibroblasts.

This study aimed to assess the acute and delayed cytotoxicity of three, popular light-cured methacrylate-based restorative resins (MRs): Charisma (C), Estelite (E), and Filtek (F), to human gingival fibroblasts in culture. Cells were grown for up to 24 h with light-cured (or pre-cured) resins. We evaluated resin cytotoxicity, redox imbalance, necrosis/apoptosis, miR-9, and heat shock protein 70 (HSP70).

Cytotoxicity and Oxidative Stress Induction by Root Canal Sealers in Human Periodontal Ligament Fibroblasts: An in vitro Study.

Introduction: The aim of the study was to investigate the cytotoxicity, the profile of cell death, and the level of oxidative stress in human periodontal ligament fibroblasts (HPdLFs) after exposure to selected root canal sealers.

Methods And Materials: Freshly mixed or set Endomethasone N (EN), RealSeal (RSEAL), Roeko Seal Automix (RSA), and Sealapex (SP) were incubated with HPdLFs. Fluorescein isothiocyanate (FITC)-annexin V (AnV) and propidium iodide (PI) staining followed by flow cytometry was used to identify the effects of the materials on cell viability and the profile of cell death.

Impact of a toothpaste with microcrystalline hydroxyapatite on the occurrence of early childhood caries: a 1-year randomized clinical trial.

The aim of this trial was to determine whether a toothpaste with microcrystalline hydroxyapatite is not inferior to a fluoride toothpaste in prevention of caries in children. This double-blinded randomized control trial compared two toothpastes regarding the occurrence of caries lesions using International Caries Detection and Assessment System (ICDAS) ≥ code 1 on the primary dentition within 336 days. The test group used a fluoride-free hydroxyapatite toothpaste three times daily while control group used a toothpaste with fluoride.

Performance of ICDAS II and fluorescence methods on detection of occlusal caries-An ex vivo study.

Background: The aim of this ex vivo study was to compare the performance of the visual ICDAS II scale and two fluorescence methods (DIAGNOdent Pen and VistaCam iX) in detecting occlusal caries lesions in permanent teeth using cone beam computed tomography (CBCT) as a reference test.

Methods: One hundred-sixty molars and premolars were qualified for the study. The visual, the DIAGNOdent Pen and VistaCam iX examinations were carried out by two examiners.

Comparison of the radiopacity of selected materials used for vital pulp therapy: An in vitro assessment.

Background: An assessment of the therapeutic effects of vital pulp treatment is based on both clinical and radiological evaluation.

Objectives: The aim of the research was a long-term (after 1, 2, 4, 6, and 8 weeks) radiological assessment of X-ray absorption by 8 selected materials used for the vital treatment of dental pulp.

Material And Methods: The materials, prepared in accordance with the manufacturers' recommendations, were placed in molds measuring 10 mm in diameter and 2 mm in thickness.

Diagnostic validity of the use of ICDAS II and DIAGNOdent pen verified by micro-computed tomography for the detection of occlusal caries lesions-an in vitro evaluation.

The aim of this study is to assess the extent of early carious lesions using the International Caries Detection and Assessment System (ICDAS) II and DIAGNOdent pen compared with microCT examination. Forty-eight molars and premolars were qualified for the study. The visual and the DIAGNOdent pen device examinations were carried out by two examiners.

A comparative assessment of the antibacterial activity of root canal sealers on 2 Actinomyces species: An in vitro study.

Background: Actinomyces species have a low virulence and pathogenicity, but under specific circumstances they may be involved in root canal and periapical tissue infections.

Objectives: The aim of the study was to investigate the antibacterial activity of various root canal sealers on standardized strains of Actinomyces.

Material And Methods: The materials tested in this study included AH Plus™ Jet (AH), Apexit® Plus (AP), Endomethasone N (EN), GuttaFlow® (GF), Hybrid Root SEAL (HB), MTA Fillapex (FL), Real® Seal (RCS), Roeko Seal Automix (RSA), Sealapex™ (SP), and Tubli-Seal™ (TS).

Effect of root canal sealers on human periodontal ligament fibroblast viability: ex vivo study.

The aim of the study was to compare ex vivo the toxic effects of six root canal sealers immediately after mixing or setting on human periodontal ligament fibroblasts (HPdLF). Freshly mixed (I group) or set (allowed to dry for 24 h) (II group) specimens of AH Plus Jet (AH), Apexit Plus (AP), MTA Fillapex (FL), GuttaFlow (GF), MetaSEAL Soft (META), and Tubli-Seal (TS) were prepared. HPdLF were exposed for 24 h to the specimens.

Evaluation of the cytotoxicity of selected conventional glass ionomer cements on human gingival fibroblasts.

Background: Dentistry materials are the most frequently used substitutes of human tissues. Therefore, an assessment of dental filling materials should cover not only their chemical, physical, and mechanical characteristics, but also their cytotoxicity.

Objectives: To compare the cytotoxic effects of 13 conventional glass ionomer cements on human gingival fibroblasts.

[Importance of dental care to maintain oral health of children and youth with type 1 diabetes].

Background: Dental care for children and youth with type 1 diabetes should rely on reliable examination of the oral cavity, early treatment of dental caries and maintenance of the treatment results. This can be achieved through regular control visits consistent with the standards, and also through following the recommendations for prevention of dental caries and periodontal disease. The aim of the study was to investigate whether dental care allows to preserve good oral health of children and youth with type 1 diabetes.

Direct Pulp Capping in Primary Molars using Mineral Trioxide Aggregate: An in Vivo Study.

Objective: To do a clinical and radiographic evaluation of the effectiveness of MTA when used as a direct pulp capping material in primary molars.

Study Design: Clinical and radiographic follow-up was performed on 30 primary molars with deep caries lesions in 30 patients from 3 to 9.75 years of age.

Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials - an in vitro study.

Introduction: Various materials are used in direct dental pulp capping method. Their biocompatibility and alkalizing abilities are of primary importance affecting therapeutic effects. The aim of this study was to evaluate and compare the cytotoxicity of various pulp-capping materials on human gingival fibroblasts and investigate the pH changes induced by these materials.

Assessment of cytotoxic potential of root canal sealers after hardening - an ex vivo study.

Aim: The aim of this study was to perform a comparative assessment of the toxic action of root canal sealers currently on the market on human gingival fibroblasts after setting.

Material/methods: The inserts with an equal quantity of set root canal sealers were transferred into 24-well culture dishes containing human gingival fibroblasts cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum (FCS). The dishes with materials were incubated at 37°C, 100% humidity and in an atmosphere of 5% CO2 for 24 h.

Antibacterial activity of selected glass ionomer cements.

Introduction: The aim of the paper was to determine the antibacterial activity of four glass ionomer cements against bacteria of the genera Streptococcus and Lactobacillus.

Material And Methods: Four capsulated glass ionomer cements were applied in the study: Fuji Triage (GC), Fuji IX (GC), Ketac Molar (3M Espe) and Ketac Silver (3M Espe). Four standard bacterial strains were used to assess the antibacterial activity of the studied cements: Streptococcus mutans, S.

Antibacterial activity of two Mineral Trioxide Aggregate materials in vitro evaluation.

Introduction: It has been documented that total removal of carious dentin, assessed according to clinical criteria, does not mean complete eradication of bacteria. For that reason in prevention of caries and pulp inflammations, application of the materials having high antibacterial properties seems to be indispensable.

Aim: Determination of antibacterial activities of two white Mineral Trioxide Aggregate (MTA) preparations against bacteria of the genus Streptococcus.

Fluoride release and antibacterial activity of selected dental materials.

The aim of the study was to assess the fluoride ion release and antibacterial activities of the glassionomer cement Fuji IX and the compomer (composite modified polyacid) Dyract AP. Fluoride ion release was measured using direct potentiometry with an Orion fluoride ion selective electrode. The measurement was carried out after 1, 4, 7, 14, 30, and 60 days of storage in phosphate buffer at pH 6.