A Simple and Scalable Chopped-Thallus Transformation Method for .

The liverwort has emerged as a valuable model for studying fundamental biological processes and the evolutionary history of land plants. -mediated transformation is widely used for genetic modification of using spores, thalli, and gemmae. While spores offer high transformation efficiency, they result in diverse genetic backgrounds due to sexual reproduction.

Optimization of Selection Agent Concentrations and Expanding G418 Utility for Gentamicin Resistance in Marchantia polymorpha.

Genetic transformation of plants is pivotal for advancing biotechnology, with success depending largely on effective selection methods. has emerged as a model plant due to its evolutionary importance, ease of manipulation, and simple genetic structure. However, inconsistent antibiotic performance and limited studies on optimal selection agent concentrations have posed challenges.