On the Unstructured Big Data Analytical Methods in Firms: Conceptual Model, Measurement, and Perception.

Firms face challenging analytical tasks at the advent of a growing amount of unstructured big data (BD). These data lead to radical shifts in their analytical strategies and market insights. Yet, the particular types of analytical methods remain in the literature still loosely scattered.

The microfluidic system for studies of carcinoma and normal cells interactions after photodynamic therapy (PDT) procedures.

This study reports on the use of a microsystem for evaluation of photodynamic therapy (PDT) procedures on the "mixed" (carcinoma-normal) cultures. Balb/3T3 (normal mouse embryo) and A549 (human lung carcinoma) cells were tested in separated and "mixed" cultures. Interactions and migration of cells cultured together were observed.

A microfluidic device with fluorimetric detection for intracellular components analysis.

An integrated microfluidic system that coupled lysis of two cell lines: L929 fibroblasts and A549 epithelial cells, with fluorescence-based enzyme assay was developed to determine β-glucocerebrosidase activity. The microdevice fabricated in poly(dimethylsiloxane) consists of three main parts: a chemical cell lysis zone based on the sheath flow geometry, a micromeander and an optical fibers detection zone. Unlike many methods described in literature that are designed to analyse intracellular components, the presented system enables to perform enzyme assays just after cell lysis process.

Evaluation of photodynamic therapy (PDT) procedures using microfluidic system.

A hybrid PDMS/glass microfluidic system for evaluation of the efficiency of photodynamic therapy is presented. 5-aminolevulinic acid (ALA) was used as a precursor of photosensitizer. The geometry of the microdevice presented in this paper enables to test different concentrations of the photosensitizer in a single assay.

Miniaturized tools and devices for bioanalytical applications: an overview.

This article presents an overview of various miniaturized devices and technologies developed by our group. Innovative, fast and cheap procedures for the fabrication of laboratory microsystems based on commercially available materials are reported and compared with well-established microfabrication techniques. The modules fabricated and tested in our laboratory can be used independently or they can be set up in different configurations to form functional measurement systems.