Automated, systematic determination of protein subcellular location using fluorescence microscopy.

Proteomics is the comprehensive study of all aspects of protein behavior. The subfield of location proteomics is concerned with the systematic analysis of the subcellular location of proteins. In order to perform high-resolution, high-throughput analysis of all protein location patterns, automation is needed both for acquisition and analysis.

Large-scale automated analysis of location patterns in randomly tagged 3T3 cells.

Location proteomics is concerned with the systematic analysis of the subcellular location of proteins. In order to perform high-resolution, high-throughput analysis of all protein location patterns, automated methods are needed. Here we describe the use of such methods on a large collection of images obtained by automated microscopy to perform high-throughput analysis of endogenous proteins randomly-tagged with a fluorescent protein in NIH 3T3 cells.