Enucleation following treatment with intravenous pentamidine for Acanthamoeba sclerokeratitis.

Purpose: To describe the course and outcome of treatment of advanced Acanthamoeba sclerokeratitis with intravenous pentamidine.

Methods: A case of advanced Acanthamoeba sclerokeratitis was resistant to conventional therapy and was treated with intravenous pentamidine. The eye was later removed due to incapacitating pain.

Combined acanthamoeba and Stenotrophomonas maltophilia keratitis treated with a conjunctival flap followed by penetrating keratoplasty.

Purpose: To describe whether a technique of treatment of a combined acanthamoeba-stenotrophomonas keratitis with a conjunctival flap followed by a penetrating keratoplasty is successful.

Methods: A case of chronic ring-shaped keratitis in which Stenotrophomonas maltophilia was isolated with corneal scrapings, and acanthamoeba cysts were found with a corneal biopsy. The infection was resistant to conventional medical treatment.

High frequency of submicroscopic hemizygous deletion is a major mechanism of loss of expression of PTEN in uveal melanoma.

Purpose: Although cytogenetic aberrations at 10q have been reported in up to 27% of uveal melanomas, the role of PTEN in the pathogenesis of uveal melanoma is largely unknown. Our aim was to determine the frequency and clinical significance of PTEN alterations in uveal melanomas.

Patients And Methods: We examined PTEN expression using immunohistochemistry in 75 sporadic uveal melanomas, with an average follow-up of 89 months.

Expression of vascular endothelial growth factor in uveal melanoma is independent of 6p21-region copy number.

Purpose: Overexpression of vascular endothelial growth factor (VEGF) and overrepresentation of the 6p region have been reported with a wide variation in uveal melanoma. The aim of the current study is to identify the frequency of copy number alteration in the 6p21 region and its correlation with the expression of VEGF in uveal melanoma.

Experimental Design: We studied 88 uveal melanomas for copy number change in the 6p region by comparative genomic hybridization and/or chromogenic in situ hybridization.

Management of medulloepithelioma of the ciliary body with brachytherapy.

Purpose: To report a case of an adult with a ciliary body mass diagnosed as a medulloepithelioma by fine-needle aspiration, confirmed by tissue biopsy, and successfully treated with brachytherapy.

Design: Interventional case report.

Methods: A 23-year-old white woman presented with a large mass originating in the ciliary body of the left eye.