A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha.

Cell-free (in vitro) expression is a robust alternative platform to the cell-based (in vivo) system for recombinant protein production. Tumor necrosis factor-alpha (TNF-α) is an effective pro-inflammatory cytokine with pleiotropic effects. The aim of the current study was de novo optimized expression of soluble and active human TNF-α by an in vitro method in an -based cell-free protein synthesis (CFPS) system and its biological activity evaluation.

De novo expression and antibacterial potential of four lactoferricin peptides in cell-free protein synthesis system.

For the first time, we produced four lactoferricin (LFcin) peptides by a cell-free () method. These short antimicrobial peptides were expressed in an cell-free protein synthesis (CFPS) system and the bioactivity of the produced peptides was demonstrated. Additionally, we designed a novel synthetic consensus peptide (ConLFcin).