Publications by authors named "Elodie Chatre"
Nucleic acid delivery requires vectorization for protection from nucleases, preventing clearance by the reticuloendothelial system, and targeting to allow cellular uptake. Nanovectors meeting the above specifications should be safe for the patient, simple to manufacture, and display long-term stability. Our nanovectors were obtained via the green process of polyelectrolyte complexation, carried out at 25 °C in water at a low shear rate using chitosan (a polycationic biocompatible polysaccharide of specific molar mass and acetylation degree) and dextran sulfate as a polyanionic biocompatible polysaccharide.
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- NLRP3-associated autoinflammatory disease is a collection of genetic disorders caused by mutations that lead to increased activity of the NLRP3 protein, which complicates diagnosis despite effective treatments available.
- The study explores 34 different NLRP3 mutations, illustrating their functional differences based on how they respond to various signals that activate them, and highlights their link to symptom severity and misdiagnosis.
- Findings reveal critical areas in the NLRP3 protein that affect its activity and response to treatments, offering new perspectives on the disorder's variability and potential directions for improving diagnosis and therapy.
Condensin shapes mitotic chromosomes by folding chromatin into loops, but whether it does so by DNA-loop extrusion remains speculative. Although loop-extruding cohesin is stalled by transcription, the impact of transcription on condensin, which is enriched at highly expressed genes in many species, remains unclear. Using degrons of Rpb1 or the torpedo nuclease Dhp1 to either deplete or displace RNAPII on chromatin in fission yeast metaphase cells, we show that RNAPII does not load condensin on DNA.
View Article and Find Full Text PDFBacteria tumble periodically, following environmental cues. Whether they can tumble near a solid surface is a basic issue for the inception of infection or mineral biofouling. Observing freely swimming Escherichia coli near and parallel to a glass surface imaged at high magnification (×100) and high temporal resolution (500 Hz), we identified tumbles as events starting (or finishing, respectively) in abrupt deceleration (or reacceleration, respectively) of the body motion.
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- Polyadenylation is a process that helps eliminate defective RNA and is important for gene expression in both eukaryotes and prokaryotes, like E. coli.
- In studies, E. coli strains without poly(A)polymerase I (PAP I) showed reduced flagellin levels and lower transcription of flagellar biosynthesis genes, suggesting that PAP I positively influences their expression.
- Despite the reduced expression of flagellar genes in the mutant strains, there were no major defects in flagella synthesis or swimming speed, although they were less motile on soft agar and more sensitive to antibiotics, hinting at PAP I's role in helping bacteria adjust to environmental challenges.
Flagellar propulsion of swimming Escherichia coli produces circling clockwise motions near planar solid surfaces. Counterclockwise motion was first reported near air-TN medium interfaces, showing that slip at the interface is a key parameter of bacterial swimming.
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