Optimized miR-124 reporters uncover differences in miR-124 expression among neuronal populations .

Introduction: Although intensively studied in the last decades, how microRNAs (miRNAs) are expressed across different cell types in the brain remains largely unknown.

Materials: To address this issue, we sought to develop optimized fluorescence reporters that could be expressed in precise cellular subsets and used to accurately quantify miR contents .

Results: Focusing on miR-124, we tested different reporter designs whose efficiency was confirmed in different settings including cell lines and primary neuronal cultures from different brain structures.