Crude Methanol Extract of Induces Apoptosis and Cell Cycle Arrest at S-Phase in Human Breast Cancer Cells.

The aim of the present study was to determine cytotoxic activity of crude methanolic extract of on breast cancer MDA-MB-231 cell line. The free radical scavenging effects of methanolic extract of were tested using DPPH method. Crude methanolic extract exhibited potential antioxidant activity with an IC value of 234.

Lack of association of GSTT1 and GSTP1 genes methylation and their expression profiles with risk of NAFLD in a sample of Iranian patients.

Unlabelled: Reactive oxygen species can affect many cellular functions through protein oxidation or initiation of the lipid peroxidation cascade that can lead to non-alcoholic fatty liver disease (NAFLD), characterized by significant lipid deposition in the hepatocytes of patients with no history of excess alcohol intakes. The present study aimed to analyze the methylation status of the antioxidative stress genes GSTT1 (glutathione S-transferase theta-1) and GSTP1 (glutathione S-transferase pi-1), and their expression profiles, in a sample population of patients with NAFLD living in South-East Iran.

Patients And Methods: Peripheral blood samples were obtained from 80 NAFLD patients and 80 healthy controls.

Promoter hypermethylation and expression profile of MGMT and CDH1 genes in oral cavity cancer.

Background: Several genetic alterations have been reported to contribute to the development of oral squamous cell carcinoma (OSCC). Methylation of CpG-islands in cancer-related genes may serve as epigenetic biomarkers for oral cancer diagnosis and prognosis. The objective of this study was to analyze methylation profile of MGMT and CDH1 genes and their link with expression activity in patients with oral cavity cancer.

Analysis of methylation patterns and expression profiles of p14ARF gene in patients with oral squamous cell carcinoma.

Aims: To analyze the promoter methylation profile and mRNA expression of the p14ARF gene in oral squamous cell carcinoma (OSCC).

Methods: Promoter methylation of the p14ARF gene was investigated by methylation-specific polymerase chain reaction in paraffin-embedded tissues from 76 patients with OSCC and 57 oral tissues used as healthy controls. Expression of p14ARF mRNA was also determined using real-time quantitative reverse-transcription PCR.