Publications by authors named "Elmarie Davoren"
Article Synopsis
- Tuberculous meningitis (TBM) not only involves Mycobacterium tuberculosis infecting the brain but also significantly affects gut microbiota, leading to systemic issues like altered immunity and metabolism.
- A study using liquid chromatography-tandem mass spectrometry analyzed urine samples from children with TBM and identified eight unique metabolites that indicate gut microbiome disruption.
- The findings underscore the connection between TBM and gut metabolism, suggesting that understanding these relationships could enhance treatment strategies and improve quality of life for affected individuals.
The aim of this study was to improve the extraction method for urinary organic acids by miniaturizing and automating the process. Currently, manual extraction methods are commonly used, which can be time-consuming and lead to variations in test results. To address these issues, we reassessed and miniaturized the in-house extraction method, reducing the number of steps and the sample-to-solvent volumes required.
View Article and Find Full Text PDFPurine and pyrimidine disorders are often difficult to diagnose. Here, we present a H-NMR analysis protocol for the quantification of purines and pyrimidines in urine to diagnose associated disorders. We describe steps for pH adjustment, sample preparation, and H-NMR analysis and data analysis.
View Article and Find Full Text PDFIntroduction: Metabolome variations have long been associated with normal hormonal fluctuations, and similar effects, related to the use of early generation synthetic hormones as a means of contraception, have also been identified.
Objective: We investigated the serum amino acid and acylcarnitine profiles induced by the use of combined oral contraceptives (COCs) consisting of Ethinylestradiol (EE) and a 4th generation progestin, Drospirenone (DRSP).
Method: Gas chromatography mass spectrometry and liquid chromatography with tandem mass spectrometry was used to identify and quantify the serum amino acids and acyl carnitine levels in 24 controls, 25 DRSP/20EE users and 26 DRSP/30EE users.