Publications by authors named "Elmar van der Wijk"
Article Synopsis
- Microbiomes are crucial for plant growth, but the genetic factors influencing their development are not well understood.
- The study analyzes the rhizosphere microbiome in a variety of wild and domesticated tomatoes, identifying gene regions that influence which bacteria are recruited, including important genes related to iron and water regulation.
- By combining microbiome research with plant genetics, the research highlights potential traits that could be used to improve plant-microbiome interactions in future breeding efforts.
Single-molecule fluorescence detection offers powerful ways to study biomolecules and their complex interactions. Here, nanofluidic devices and camera-based, single-molecule Förster resonance energy transfer (smFRET) detection are combined to study the interactions between plant transcription factors of the auxin response factor (ARF) family and DNA oligonucleotides that contain target DNA response elements. In particular, it is shown that the binding of the unlabeled ARF DNA binding domain (ARF-DBD) to donor and acceptor labeled DNA oligonucleotides can be detected by changes in the FRET efficiency and changes in the diffusion coefficient of the DNA.
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- Auxin, a plant hormone, regulates the expression of thousands of genes through AUXIN RESPONSE transcription FACTORs (ARFs), which bind to specific DNA sequences called AuxRE motifs.
- Research revealed that ARF1 can form higher-affinity bonds to DNA with an additional hydrogen bond, enhancing its binding potential significantly.
- The study also found that different ARF subfamilies interact variably with DNA, which helps explain the diverse functions of auxin-responsive elements in plant gene regulation.