Sulfur amino acid supplementation displays therapeutic potential in a C. elegans model of Duchenne muscular dystrophy.

Mutations in the dystrophin gene cause Duchenne muscular dystrophy (DMD), a common muscle disease that manifests with muscle weakness, wasting, and degeneration. An emerging theme in DMD pathophysiology is an intramuscular deficit in the gasotransmitter hydrogen sulfide (HS). Here we show that the C.

Loss of physical contact in space alters the dopamine system in .

Progressive neuromuscular decline in microgravity is a prominent health concern preventing interplanetary human habitation. We establish functional dopamine-mediated impairments as a consistent feature across multiple spaceflight exposures and during simulated microgravity in . Animals grown continuously in these conditions display reduced movement and body length.

The Likelihood of Early Guilty Pleas Following Digitally Recorded Victim Statements for Family Violence.

This study follows 4715 Family Harm cases for which charges are laid (from around 15,000 events from 2018-2020). Comparisons are made between cases where a digitally recorded victim video statement (VVS) is taken to those who (1) make a written statement, (2) refuse to make any statement and (3) present at the public counter and make a written statement. Findings indicate that VVS increases the rates of an early guilty plea by 95% (OR = 1.

as a Model System for Duchenne Muscular Dystrophy.

The nematode worm has been used extensively to enhance our understanding of the human neuromuscular disorder Duchenne Muscular Dystrophy (DMD). With new arising clinically relevant models, technologies and treatments, there is a need to reconcile the literature and collate the key findings associated with this model.

Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B.

Tyrosine kinase inhibitors (TKIs) targeting BCR-ABL1 have revolutionized therapy for chronic myeloid leukemia (CML), paving the way for clinical development in other diseases. Despite success, targeting leukemic stem cells and overcoming drug resistance remain challenges for curative cancer therapy. To identify drivers of kinase-independent TKI resistance in CML, we performed genome-wide expression analyses on TKI-resistant versus sensitive CML cell lines, revealing a nuclear factor-kappa B (NF-κB) expression signature.

Mitochondrial hydrogen sulfide supplementation improves health in the Duchenne muscular dystrophy model.

Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder characterized by progressive muscle degeneration and weakness due to mutations in the dystrophin gene. The symptoms of DMD share similarities with those of accelerated aging. Recently, hydrogen sulfide (HS) supplementation has been suggested to modulate the effects of age-related decline in muscle function, and metabolic HS deficiencies have been implicated in affecting muscle mass in conditions such as phenylketonuria.

Detection of hydroacoustic signals on a fiber-optic submarine cable.

A ship-based seismic survey was conducted close to a fiber-optic submarine cable, and 50 km-long distributed acoustic sensing (DAS) recordings with air-gun shots were obtained for the first time. We examine the acquired DAS dataset together with the co-located hydrophones to investigate the detection capability of underwater acoustic (hydroacoustic) signals. Here, we show the hydroacoustic signals identified by the DAS measurement characterizing in frequency-time space.

Molecular Muscle Experiment: Hardware and Operational Lessons for Future Astrobiology Space Experiments.

Biology experiments in space seek to increase our understanding of what happens to life beyond Earth and how we can safely send life beyond Earth. Spaceflight is associated with many (mal)adaptations in physiology, including decline in musculoskeletal, cardiovascular, vestibular, and immune systems. Biological experiments in space are inherently challenging to implement.

A yellowness index for use in dentistry.

Objectives: To develop an equation that predicts the perceptual yellowness of teeth.

Methods: A large set of new psychophysical yellowness data were generated from an experiment where 500 participants each ranked a set of 58 shade guide samples. Two existing equations (WIO and b*) and one new equation (YIO) were evaluated by comparing their values for the 58 shade guide tabs with the psychophysical data.

An Experimental Review of Optical Coherence Tomography Systems for Noninvasive Assessment of Hard Dental Tissues.

Optical coherence tomography (OCT) is a noninvasive, high-resolution, cross-sectional imaging technique. To date, OCT has been demonstrated in several areas of dentistry, primarily using wavelengths around 1,300 nm, low numerical aperture (NA) imaging lenses, and detectors insensitive to the polarization of light. The objective of this study is to compare the performance of three commercially available OCT systems operating with alternative wavelengths, imaging lenses, and detectors for OCT imaging of dental enamel.

Feasibility of a Real-Time Clinical Augmented Reality and Artificial Intelligence Framework for Pain Detection and Localization From the Brain.

Background: For many years, clinicians have been seeking for objective pain assessment solutions via neuroimaging techniques, focusing on the brain to detect human pain. Unfortunately, most of those techniques are not applicable in the clinical environment or lack accuracy.

Objective: This study aimed to test the feasibility of a mobile neuroimaging-based clinical augmented reality (AR) and artificial intelligence (AI) framework, CLARAi, for objective pain detection and also localization direct from the patient's brain in real time.

Three dimensional photoacoustic tomography in Bayesian framework.

The image reconstruction problem (or inverse problem) in photoacoustic tomography is to resolve the initial pressure distribution from detected ultrasound waves generated within an object due to an illumination by a short light pulse. Recently, a Bayesian approach to photoacoustic image reconstruction with uncertainty quantification was proposed and studied with two dimensional numerical simulations. In this paper, the approach is extended to three spatial dimensions and, in addition to numerical simulations, experimental data are considered.

European Organization for Caries Research Workshop: Methodology for Determination of Potentially Available Fluoride in Toothpastes.

Toothpastes are the most universally accepted form of fluoride delivery for caries prevention. To provide anti-caries benefits, they must be able to release fluoride during the time of tooth brushing or post brushing into the oral cavity. However, there is no standard accepted procedure to measure how much fluoride in a toothpaste may be (bio) available for release.

Evaluation of colorimetric indices for the assessment of tooth whiteness.

Objectives: To evaluate the performance of existing equations that measure perceptual whiteness of teeth.

Methods: Three new psychophysical experiments were conducted and combined with two previously published experiments to form a large set of data to test performance of whiteness indices. Three whiteness indices (WIC, WIO, WI,) were compared with regard to their ability to measure perceived whiteness.

Automatic detection and classification of dental fluorosis in vivo using white light and fluorescence imaging.

Objectives: To assess a novel method of automatic fluorosis detection and classification from white light and fluorescent images.

Methods: Dental images from 1,729 children living in two fluoridated and two non-fluoridated UK cities were utilised. A novel detection and classification algorithm was applied to each image and TF scores were obtained using thresholding criteria.

Digital epidemiology - Calibrating readers to score dental images remotely.

Background: There is growing interest to use digital photographs in dental epidemiology. However, the reporting of procedures and metric-based performance outcomes from training to promote data quality prior to actual scoring of digital images has not been optimal.

Methods: A training study was undertaken to assess training methodology and to select a group of scorers to assess images for dental fluorosis captured during the 2013-2014 National Health and Nutrition Examination Survey (NHANES).

Brain Functional Changes before, during, and after Clinical Pain.

This study used an emerging brain imaging technique, functional near-infrared spectroscopy (fNIRS), to investigate functional brain activation and connectivity that modulates sometimes traumatic pain experience in a clinical setting. Hemodynamic responses were recorded at bilateral somatosensory (S1) and prefrontal cortices (PFCs) from 12 patients with dentin hypersensitivity in a dental chair before, during, and after clinical pain. Clinical dental pain was triggered with 20 consecutive descending cold stimulations (32° to 0°C) to the affected teeth.

Statistical independence in nonlinear model-based inversion for quantitative photoacoustic tomography.

The statistical independence between the distributions of different chromophores in tissue has previously been used for linear unmixing with independent component analysis (ICA). In this study, we propose exploiting this statistical property in a nonlinear model-based inversion method. The aim is to reduce the sensitivity of the inversion scheme to errors in the modelling of the fluence, and hence provide more accurate quantification of the concentration of independent chromophores.

Red fluorescence imaging for dental plaque detection and quantification: pilot study.

The red fluorescence of dental plaque originating from porphyrins in oral bacteria may allow visualization, detection, and scoring of plaque without disclosing agents. Two studies were conducted. The first included 24 healthy participants who abstained from oral hygiene for 24 h.

Role of microbial biofilms in the maintenance of oral health and in the development of dental caries and periodontal diseases. Consensus report of group 1 of the Joint EFP/ORCA workshop on the boundaries between caries and periodontal disease.

Background And Aims: The scope of this working group was to review (1) ecological interactions at the dental biofilm in health and disease, (2) the role of microbial communities in the pathogenesis of periodontitis and caries, and (3) the innate host response in caries and periodontal diseases.

Results And Conclusions: A health-associated biofilm includes genera such as Neisseria, Streptococcus, Actinomyces, Veillonella and Granulicatella. Microorganisms associated with both caries and periodontal diseases are metabolically highly specialized and organized as multispecies microbial biofilms.