Activation of hTREK-1 by polyunsaturated fatty acids involves direct interaction.

TREK-1 is a mechanosensitive channel activated by polyunsaturated fatty acids (PUFAs). Its activation is supposed to be linked to changes in membrane tension following PUFAs insertion. Here, we compared the effect of 11 fatty acids and ML402 on TREK-1 channel activation using the whole cell and the inside-out configurations of the patch-clamp technique.

From MS/MS library implementation to molecular networks: Exploring oxylipin diversity with NEO-MSMS.

Oxylipins, small polar molecules derived from the peroxidation of polyunsaturated fatty acids (PUFAs), serve as biomarkers for many diseases and play crucial roles in human physiology and inflammation. Despite their significance, many non-enzymatic oxygenated metabolites of PUFAs (NEO-PUFAs) remain poorly reported, resulting in a lack of public datasets of experimental data and limiting their dereplication in further studies. To overcome this limitation, we constructed a high-resolution tandem mass spectrometry (MS/MS) dataset comprising pure NEO-PUFAs (both commercial and self-synthesized) and in vitro free radical-induced oxidation of diverse PUFAs.

A new rolling forecasting framework using Microsoft Power BI for data visualization: A case study in a pharmaceutical industry.

Context And Objectives: Demand forecasting is a vital step for production planning and consequently, for supply chain efficiency, especially for the pharmaceutical (pharma) supply chain due to its unique characteristics. Numerous models and techniques that are proposed in the literature but little in concrete and generic framework to forecasting process, mainly for pharmaceutical supply chain. Unlike studies in the literature, this study not only perfectly predict the sales of a pharma manufacturer, but also visualize the results via a developed dashboard using modern information technology and business intelligence.

Morphological and molecular characterization of Chloromyxum dasyatidis n. sp. (Myxosporea: Chloromyxidae) in the common stingray Dasyatis pastinaca (Linnaeus) from Tunisian waters (Central Mediterranean Sea).

A new species of Chloromyxum Mingazzini, 1890, C. dasyatidis n. sp.

Fatal invasive gastric mucormycosis: Two case reports.

Mucormycosis is a fungal infection affecting most commonly immunocompromised patients. Hereby, we report two cases: the first one is about a 61-year-old female with diabetes who presented with vomiting. The upper gastrointestinal endoscopy showed a budding grayish process which corresponded to an invasive mucormycosis in histology.

Fortuitous discovery of isolated tuberculosis of the tenth and eleventh costotransverse joints: A case report.

Involvement of posterior elements of the spine in spinal tuberculosis is rare. We report a case of a 56-year-old woman who presented with asymptomatic tuberculosis of the tenth and eleventh costotransverse joints. The latter was discovered with a CT scan made to look for a pulmonary embolism.

Myxidium tunisiensis n. sp. (Myxosporea: Myxidiidae) infecting the rough skate Raja radula Delaroche, 1908 (Rajiformes: Rajidae) from North East Tunisia.

A new marine myxosporean, Myxidium tunisiensis n. sp. (Myxosporea: Myxidiidae), is described infecting the gallbladder of the rough skate Raja radula Delaroche, 1908 (Chondrichthyes: Rajidae) harvested from the Bay of Bizerte, Tunisia.

Six-minute walk distance equation in children and adolescents with obesity.

Aim: To develop a 6-minute walk-distance (6MWD) reference equation for children and adolescents with obesity, and to assess its determinant factors.

Methods: A cross-sectional study using mixed linear and multiple regression models was conducted in 497 children (235 girls and 262 boys) aged 6-18 years with a body mass index (BMI) greater than 97th percentile. Anthropometrics and a 6-minute walk test (6MWT) were performed.

Floating Forearm with Terrible Triad Injury of the Elbow: A Case Report.

Introduction: Floating forearm is rare. Simultaneous terrible triad injury of the elbow is even rarer.

Case Report: A 47-year-old male patient presented with concurrent dorsal perilunate dislocation of the left wrist combined with a terrible triad injury of the left elbow after a fall from the second floor.

Psychometric data concerning the Positive and Negative Affect Schedule - Direction (PANAS-D) in athletes in Arabic-Tunisian language supporting a 2-factor structure of its short modified version.

The Positive and Negative Affect Schedule - Direction (PANAS-D) questionnaire was translated from the French version developed by Nicolas and coworkers into Arabic-Tunisian language and administered to a sample of 519 athletes (mean age 19.43±3.78 years; 230 male, 229 female; 75 competing at international level, 287 at national level, 130 at regional level, and 27 at local level).

Congenital heart disease in 37,294 births in Tunisia: birth prevalence and mortality rate.

Aim: To investigate the previously unknown birth incidence, treatment, and mortality of children with congenital heart disease in Tunisia.

Methods: We undertook a retrospective review of medical records of all patients who were born in 2010 and 2011, and were diagnosed in Sfax (Tunisia) with congenital heart defect.

Results: Among 37,294 births, 255 children were detected to have congenital heart disease, yielding a birth incidence of 6.