Models of utricular bouton afferents: role of afferent-hair cell connectivity in determining spike train regularity.

Vestibular bouton afferent terminals in turtle utricle can be categorized into four types depending on their location and terminal arbor structure: lateral extrastriolar (LES), striolar, juxtastriolar, and medial extrastriolar (MES). The terminal arbors of these afferents differ in surface area, total length, collecting area, number of boutons, number of bouton contacts per hair cell, and axon diameter (Huwe JA, Logan CJ, Williams B, Rowe MH, Peterson EH. 113: 2420-2433, 2015).

Quantifying utricular stimulation during natural behavior.

The use of natural stimuli in neurophysiological studies has led to significant insights into the encoding strategies used by sensory neurons. To investigate these encoding strategies in vestibular receptors and neurons, we have developed a method for calculating the stimuli delivered to a vestibular organ, the utricle, during natural (unrestrained) behaviors, using the turtle as our experimental preparation. High-speed digital video sequences are used to calculate the dynamic gravito-inertial (GI) vector acting on the head during behavior.

Mechanical properties and consequences of stereocilia and extracellular links in vestibular hair bundles.

Although knowledge of the fine structure of vestibular hair bundles is increasing, the mechanical properties and functional significance of those structures remain unclear. In 2004, Bashtanov and colleagues reported the contribution of different extracellular links to bundle stiffness. We simulated Bashtanov's experimental protocol using a three-dimensional finite element bundle model with geometry measured from a typical striolar hair cell.

Computational models of hair cell bundle mechanics: III. 3-D utricular bundles.

Six utricular hair bundles from a red-eared turtle are modeled using 3-D finite element analysis. The mechanical model includes shear deformable stereocilia, realignment of all forces during force load increments, and tip and lateral link inter-stereocilia connections. Results show that there are two distinct bundle types that can be separated by mechanical bundle stiffness.

Quantitative analysis of stereociliary arrays on vestibular hair cells.

We have developed a method for quantifying the number, spacing, and distribution of stereocilia on the apical surface of hair cells using spatial autocorrelation analysis and statistics for directional data. Here, we illustrate the method using idealized hair bundles, and we apply it to scanning micrographs of turtle hair cells from the utricle and posterior canal, and to freeze-fracture preparations of bullfrog saccule. The analysis suggests three common features of stereociliary bundles.

Are There Parallel Channels in the Vestibular Nerve?

A popular concept in neurobiology is that sensory information is transmitted to the central nervous system over parallel channels of neurons that play different functional roles. But alternative organizing schemes are possible, and it is useful to ask whether some other framework might better account for the diversity of vestibular primary afferents.

Patterns of olfactory projections in the desert iguana, Dipsosaurus dorsalis.

The neural organization of the olfactory system in the desert iguana, Dipsosaurus dorsalis, has been investigated by using the Fink-Heimer technique to trace the efferents of the main and accessory olfactory bulbs, and Golgi preparations to determine the spatial relations between olfactory afferents and neurons in the primary olfactory centers. The accessory olfactory bulb projects to the ipsilateral nucleus sphericus via the accessory olfactory tract. The main olfactory bulb projects to the ipsilateral telen-cephalon via four tracts.