Genome Analysis of Melon Necrotic Spot Virus Incursions and Seed Interceptions in Australia.

Melon necrotic spot virus (MNSV) was detected in field-grown (rockmelon) and (watermelon) plants in the Sunraysia district of New South Wales and Victoria, Australia, in 2012, 2013, and 2016, and in two watermelon seed lots tested at the Australian border in 2016. High-throughput sequencing was used to generate near full-length genomes of six isolates detected during the incursions and seed testing. Phylogenetic analysis of the genomes suggests that there have been at least two incursions of MNSV into Australia and none of the field isolates were the same as the isolates detected in seeds.