Publications by authors named "Ella C Lodewyk"
Article Synopsis
- * The study introduces Sensor-seq, a high-throughput platform that allows for the design and identification of aTF biosensors that can bind to a variety of non-native ligands, testing 17,737 variants of aTF TtgR against multiple compounds.
- * Sensor-seq successfully finds biosensors with high performance for various ligands and showcases their practical use through cell-free detection systems for specific drugs, enhancing the ability to create new biosensors beyond natural limitations.
Article Synopsis
- Allosteric transcription factors (aTFs) are important tools for detecting various molecules but designing them to work with new compounds is difficult due to potential disruptions in their allosteric properties.
- The researchers developed a high-throughput platform called Sensor-seq that screened over 17,000 variants of the aTF TtgR to find biosensors that can bind to a range of non-native and native ligands.
- The study resulted in the identification of new biosensors with excellent specificity and effectiveness, and practical applications were demonstrated through cell-free detection systems for naltrexone and quinine.
