Cutaneous tuberculosis after mesotherapy: report of six cases.

Introduction: Cutaneous tuberculosis as a result of a needle injection is a rare event; it generally occurs among medical and laboratory personnel and among patients receiving percutaneous treatment.

Objective: Six patients are presented who developed cutaneous tuberculosis after mesotherapy cosmetic treatment.

Material And Methods: One to four months after injection of an unknown product as treatment for obesity and cellulites, five women and a man developed papules, nodules and drainage of wax like material at the inoculated sites; this was interpreted clinically as a non tuberculous mycobacterium infection.

[Study of rifampin and dapsone resistance in three patients with recurring leprosy].

Objective: To detect the presence of rifampin- and dapsone-resistant strains of Mycobacterium leprae in three patients with recurring leprosy and clinically-suspected antimicrobial resistance through molecular techniques.

Methods: A retrospective, descriptive study was conducted of three multibacillary patients at the "Agua de Dios" Sanitarium in Cundinamarca, Colombia, that presented leprosy relapses that were documented by medical history, bacilloscopy, and biopsy. Biopsies were taken of the skin lesions and the bacteria were subject to DNA extraction and purification.

[Active search for leprosy and other skin diseases in school children from Agua de Dios, Colombia].

Objective: Actively searching for leprosy, other skin diseases and BCG vaccination scars amongst school children from Agua de Dios, the municipality having the highest prevalence of leprosy in Colombia.

Methods: A clinical examination of the children was carried out by nurses, interns, general practitioners and experts on leprosy. Skin smear tests and skin biopsies were performed when the clinical findings suggested leprosy.

Detection and serotyping of Streptococcus pneumoniae from nasopharyngeal samples by PCR-based multiplex assay.

We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique can be used to study the epidemiology of pneumococcal colonization and the effect of conjugate vaccines.