Publications by authors named "Elke P F Klerkx"
Homologous chromosome pairing is a prerequisite to establish physical linkage between homologs, which is critical for faithful chromosome segregation during meiosis I. The establishment of pairing is genetically separable from subsequent synapsis, defined as stabilization of pairing by the synaptonemal complex (SC). The underlying mechanism of presynaptic pairing is poorly understood.
View Article and Find Full Text PDFThe vaccinia-related kinases (VRKs) are highly conserved throughout the animal kingdom and phosphorylate several chromatin proteins and transcription factors. In early Caenorhabditis elegans embryos, VRK-1 is required for proper nuclear envelope formation. In this work, we present the first investigation of the developmental role of VRKs by means of a novel C.
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- Nuclear pore complexes (NPCs) facilitate transport between the nucleus and cytoplasm in eukaryotic cells, and are essential for gene expression regulation.
- The vertebrate protein Nup35, which is involved in NPC assembly and nuclear envelope (NE) assembly, was studied through a mutation in C. elegans called npp-19(tm2886).
- This mutation leads to chromosome missegregation and nuclear defects at high temperatures during embryogenesis, indicating that Nup35 is crucial for NE function and stability during early cell divisions.
Barrier-to-autointegration factor (BAF) is an essential, highly conserved, metazoan protein. BAF interacts with LEM (LAP2, emerin, MAN1) domain-carrying proteins of the inner nuclear membrane. We analyzed the in vivo function of BAF in Caenorhabditis elegans embryos using both RNA interference and a temperature-sensitive baf-1 gene mutation and found that BAF is directly involved in nuclear envelope (NE) formation.
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