Nile red staining for rapid screening of plastic-suspect particles in edible seafood tissues.

Concerns regarding microplastic (MP) contamination in aquatic ecosystems and its impact on seafood require a better understanding of human dietary MP exposure including extensive monitoring. While conventional techniques for MP analysis like infrared or Raman microspectroscopy provide detailed particle information, they are limited by low sample throughput, particularly when dealing with high particle numbers in seafood due to matrix-related residues. Consequently, more rapid techniques need to be developed to meet the requirements of large-scale monitoring.

Spatial distribution of atmospheric microplastics in bulk-deposition of urban and rural environments - A one-year follow-up study in northern Germany.

Atmospheric microplastic deposition rates play a crucial role for calculating the input of microplastics in the environment and to further understand pollution patterns. In this study, the spatial and temporal distribution of atmospheric microplastic particles in urban and rural areas of Northern Germany was investigated. Therefore, eleven structurally diverse locations in Hamburg and Mecklenburg-Western Pomerania were equipped with bulk-deposition samplers in triplicates and sampled monthly between August 2019 and July 2020.

Abundance and Distribution of Microplastics in Invertebrate and Fish Species and Sediment Samples along the German Wadden Sea Coastline.

Monitoring strategies are becoming increasingly important as microplastic contamination increases. To find potentially suitable organisms and sites for biota monitoring in the German Wadden Sea, we collected invertebrates ( = 1585), fish ( = 310), and sediment cores ( = 12) at 10 sites along the coast of Lower Saxony between 2018 and 2020. For sample processing of biota, the soft tissue was digested and the sediment samples additionally underwent a subsequent density separation step.

Quantification and characterization of microplastics in surface water samples from the Northeast Atlantic Ocean using laser direct infrared imaging.

15 filtration samples were collected at eight locations onboard the RV Sonne (cruise SO279 in 2020) from 6 m water depth using a fractionated stainless-steel filtration unit. The size fraction > 300 μm was visually examined and potential microplastic particles were analyzed by ATR-FTIR spectroscopy. The treatment of size class 20 μm < d < 300 μm was based on enzymatic-oxidative microwave-assisted "one-pot" matrix digestion in conjunction with analysis of the microplastics by time-efficient LDIR imaging.

Microplastics detected in cirrhotic liver tissue.

Background: The contamination of ecosystem compartments by microplastics (MPs) is an ubiquitous problem. MPs have been observed in mice tissues, and recently in human blood, stool and placenta. However, two aspects remain unclear: whether MPs accumulate in peripheral organs, specifically in the liver, and if liver cirrhosis favours this process.

Microplastic concentrations, characteristics, and fluxes in water bodies of the Tollense catchment, Germany, with regard to different sampling systems.

The widespread presence of microplastics in multiple environmental compartments has largely been demonstrated. Assessing the ecological risk that microplastics pose is, at the present stage, hindered due to methodical differences. Moreover, different methods hamper meaningful comparisons between studies and data on microplastics <300 μm is scarce.

Microplastics in lakeshore and lakebed sediments - External influences and temporal and spatial variabilities of concentrations.

Microplastics have been predominantly studied in marine environments compared to freshwater systems. However, the number of studies analyzing microplastic concentrations in water and sediment within lakes and rivers are increasing and are of utmost importance as freshwaters are major pathways for plastics to the oceans. To allow for an adequate risk assessment, detailed knowledge concerning plastic concentrations in different environmental compartments of freshwaters are necessary.

Anthropogenic litter in freshwater environments - Study on lake beaches evaluating marine guidelines and aerial imaging.

Studies on macroplastic pollution in freshwater systems are rare compared to the marine environment. Nevertheless, freshwater systems are worthy to be equally investigated as they are pathways of plastic to the ocean and lakes may act as (temporary) sinks. The aim of this study was to identify sources for plastics and influences on its distribution in a limnic environment.

Microplastics in a deep, dimictic lake of the North German Plain with special regard to vertical distribution patterns.

The investigation of microplastics (MPs) in freshwater has received increased attention within the last decade. To date, sampling is mainly conducted at the surface of both rivers and lakes and only a few studies assessed the vertical distribution of MPs in the water column of freshwater bodies. To contribute to the understanding of MP pollution in the water column of freshwater lakes, this study evaluated the vertical profile of MPs in Lake Tollense considering particles between 63 and 5000 μm in size.

Nile red staining in microplastic analysis-proposal for a reliable and fast identification approach for large microplastics.

A variety of methods concerning the identification of microplastics in environmental samples exist. While visual identification is often used, implemented easily, and cost-efficient but implying biased results, spectroscopic or chromatographic approaches are reliable but time-consuming and need specific equipment. Nile red staining is an available alternative and complement method for identifying microplastics.

On the representativeness of pump water samples versus manta sampling in microplastic analysis.

To broaden the understanding of sources, pathways and sinks for microplastic pollution in the environment, the exact and representative determination of pollution levels is crucial. Still, sampling techniques differ greatly between studies and the influence of these differences is not fully understood. Thus, we evaluate the representativeness of manta trawling and pump sampling for microplastics in a freshwater lake.

Microplastic abundance in atmospheric deposition within the Metropolitan area of Hamburg, Germany.

Only few studies investigated the input of microplastic particles via the atmosphere, so far. Here, we present results on microplastic concentrations in the atmospheric deposition in the metropolitan region of Hamburg. In total, six investigation sites were equipped with three bulk precipitation samplers each and sampled biweekly over 12 weeks (12/17-03/18).

Microplastic analysis in the South Funen Archipelago, Baltic Sea, implementing manta trawling and bulk sampling.

Microplastic contamination in surface waters of the South Funen Archipelago in Denmark was assessed. Therefore, ten manta trawls were conducted in June 2015. Moreover, 31 low-volume bulk samples were taken to evaluate, whether consistent results in comparison to the net-based approach can be obtained.

Microplastic in beach sediments of the Isle of Rügen (Baltic Sea) - Implementing a novel glass elutriation column.

To extent the understanding on microplastics in the marine environment we performed a case study at four beaches on the Isle of Rügen considering abundance and spatial distribution of microplastics in beach sediments. For the analysis, density separation via a glass elutriation column was implemented. In advance, efficiencies were tested for two polymers, being not buoyant in water.

Marine litter abundance and distribution on beaches on the Isle of Rügen considering the influence of exposition, morphology and recreational activities.

The abundance, weight and composition of marine debris were determined at the northwest coast of the Isle of Rügen in 2015. A total number of 1115 macrolitter items were registered, resulting in an abundance of 304±88.96 items per 100m of beach length and therefore being greater than the abundances found for other beaches at the Baltic Sea.

Microplastic pollution in lakes and lake shoreline sediments - A case study on Lake Bolsena and Lake Chiusi (central Italy).

Rivers and effluents have been identified as major pathways for microplastics of terrestrial sources. Moreover, lakes of different dimensions and even in remote locations contain microplastics in striking abundances. This study investigates concentrations of microplastic particles at two lakes in central Italy (Lake Bolsena, Lake Chiusi).

Analysis of somatostatin receptor 2A immunohistochemistry, RT-qPCR, and in vivo PET/CT data in patients with pancreatic neuroendocrine neoplasm.

Objective: Gallium 68 somatostatin receptor (SSTR) positron emission tomography/computed tomography (PET/CT) is one of the most sensitive imaging methods for pancreatic neuroendocrine tumors. The aim of the study was to correlate the receptor density generated from the static PET/CT (maximum standard uptake values [SUVmax], mean standard uptake values [SUVmean]) with subtype 2A SSTR (SSTR2A) immunohistochemistry and reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) gene-expression data.

Methods: Thirty-nine tumor specimens (17 primary pancreatic tumors [PTs], 22 metastases [MTS]) of 19 patients with PET/CT scans preoperatively were evaluated.