Specific Features of the Proteomic Response of Thermophilic Bacterium to Terahertz Irradiation.

Studying the effects of terahertz (THz) radiation on the proteome of temperature-sensitive organisms is limited by a number of significant technical difficulties, one of which is maintaining an optimal temperature range to avoid thermal shock as much as possible. In the case of extremophilic species with an increased temperature tolerance, it is easier to isolate the effects of THz radiation directly. We studied the proteomic response to terahertz radiation of the thermophilic , persisting under wide temperature fluctuations with a 60 °C optimum.

Heterologous Expression of Extracellular Proteinase pAsPs of in .

Neutral protease pAsPs gene was obtained by sequence optimization of NpI protease from pAsPs was for the first time integrated in the genome of yeast strain T07, and then produced in a 5 L bioreactor with an enzyme yield of 150,800 U/mL of culture liquid towards casein. The specific activity of the pAsPs was 7,657,000 U/mg toward casein, 2320 U/mg toward hemoglobin, and 25,344 U/mg toward azocasein per 1 mg of the protein. The enzyme was found to be inhibited by Cu.

Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07.

Xylanases (EC 3.2.1.

Performance of the first commercial dual resistance assay, AmpliSens -ML/FQ-Resist-FL, for detection of potential macrolide and quinolone resistance-associated mutations and prevalence of resistance mutations in St. Petersburg, Russia.

Objectives: Antimicrobial resistance in (MG) is a poorly surveyed and controlled global health concern. We evaluated the first commercial dual resistance assay, AmpliSens -ML/FQ-Resist-FL assay, for detection of potential macrolide and quinolone resistance-associated mutations (MRAMs and QRAMs, respectively) and estimated the prevalence of these mutations in MG in St. Petersburg, Russia.

Mobile Colistin Resistance Genetic Determinants of Non-Typhoid Isolates from Russia.

Polymyxin resistance, determined by genes located on plasmid DNA, currently poses a high epidemiological threat. Non-typhoid (NTS) are one of the key pathogens causing diarrheal diseases. Here, we report the isolation and whole genome sequencing of multidrug colistin-resistant/susceptible isolates of non-typhoid serovars carrying genes.

Clinical evaluation of commercial PCR assays for antimicrobal resistance in Mycoplasma genitalium and estimation of resistance-mediated mutation prevalence in Moscow and Moscow region.

Mycoplasma genitalium is a widespread sexually transmitted infection (STI) with growing rate of antimicrobials resistance. In our study, 137 vaginal and 131 urethral M. genitalium-positive swabs were sequentially collected through the work of Reference Center for STI during 2019.