Publications by authors named "Elizabeth Zoeller"
Article Synopsis
- - The TREAT-AD program aims to create reliable tools for testing hypotheses related to Alzheimer's disease, emphasizing the need for validated research antibodies used in experiments involving drug targets.
- - Researchers assessed several commercial antibodies targeting specific proteins (Moesin, CD44, Midkine, and sFRP-1) using Western blot analysis on brain tissues from a mouse model with Alzheimer's pathology.
- - The study found significant increases in the expression of these target proteins in the Alzheimer's model compared to control mice, confirming the antibodies' effectiveness for future research on Alzheimer's therapeutics.
The spleen tyrosine kinase (SYK) and high affinity immunoglobulin epsilon receptor subunit gamma (FCER1G) interaction has a major role in the normal innate and adaptive immune responses, but dysregulation of this interaction is implicated in several human diseases, including autoimmune disorders, hematological malignancies, and Alzheimer's Disease. Development of small molecule chemical probes could aid in studying this pathway both in normal and aberrant contexts. Herein, we describe the miniaturization of a time-resolved fluorescence resonance energy transfer (TR-FRET) assay to measure the interaction between SYK and FCER1G in a 1536-well ultrahigh throughput screening (uHTS) format.
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- The SYK and FCER1G proteins work together in the immune system, but problems with their interaction can lead to diseases like autoimmune issues and Alzheimer's.
- Scientists developed a special test to measure how these proteins interact, making it easier to test lots of different compounds at once.
- They found that a compound called hematoxylin can stop the interaction between SYK and FCER1G, which is exciting because it could help in finding new treatments.
Article Synopsis
- Collective invasion is a significant way that solid tumors metastasize, involving groups of cells that include both leader and follower cells with varying roles.
- Researchers analyzed gene expression differences in leader and follower cells from a lung cancer cell line, discovering 14 mutations that are more common in either group, linking genetic differences to how these cells behave.
- Two specific mutations were studied, showing that ARP3 helps leader cells move more effectively, while KDM5B prevents overly cooperative behavior among cells, emphasizing the importance of genetic diversity in cancer invasion strategies.
The KDM5/JARID1 family of Fe(II)- and α-ketoglutarate-dependent demethylases remove methyl groups from tri- and dimethylated lysine 4 of histone H3. Accumulating evidence from primary tumors and model systems supports a role for KDM5A (JARID1A/RBP2) and KDM5B (JARID1B/PLU1) as oncogenic drivers. The KDM5 family is unique among the Jumonji domain-containing histone demethylases in that there is an atypical insertion of a DNA-binding ARID domain and a histone-binding PHD domain into the Jumonji domain, which separates the catalytic domain into two fragments (JmjN and JmjC).
View Article and Find Full Text PDFObjective: The aim of this study was to investigate inherent in vitro permeability, metabolism, and cytotoxicity of idebenone - an active used to protect skin as an anti-aging agent - and compare it to idebenone linoleate.
Methods: Idebenone and idebenone linoleate were investigated in pig ear skin and melanoma (B16: F10 mouse) cells. Diffusion experiments were conducted at 37 degrees C (bath temperature) using Franz diffusion cells.