Single-cell and spatial transcriptomics identify a macrophage population associated with skeletal muscle fibrosis.

Macrophages are essential for skeletal muscle homeostasis, but how their dysregulation contributes to the development of fibrosis in muscle disease remains unclear. Here, we used single-cell transcriptomics to determine the molecular attributes of dystrophic and healthy muscle macrophages. We identified six clusters and unexpectedly found that none corresponded to traditional definitions of M1 or M2 macrophages.

Single-cell and spatial transcriptomics identify a macrophage population associated with skeletal muscle fibrosis.

The monocytic/macrophage system is essential for skeletal muscle homeostasis, but its dysregulation contributes to the pathogenesis of muscle degenerative disorders. Despite our increasing knowledge of the role of macrophages in degenerative disease, it still remains unclear how macrophages contribute to muscle fibrosis. Here, we used single-cell transcriptomics to determine the molecular attributes of dystrophic and healthy muscle macrophages.

Effect of biomaterials hydrophobicity and roughness on biofilm development.

Most hospitalized patients are carriers of biomedical devices. Infections associated with these devices cause great morbidity and mortality, especially in patients in intensive care units. Numerous strategies have been designed to prevent biofilm development on biodevices.

Food insecurity among Latin American recent immigrants in Toronto.

Food security is an important social determinant of health. The 2004 Canadian Community Health Survey, Cycle 2.2 reported high prevalence of food insecurity among low income households and those formed by recent immigrants.

[Validation of a household food security scale in Antioquia, Colombia].

Objective: To adapt and validate in households of Antioquia, Colombia, a food security scale previously applied in households of Caracas, Venezuela.

Material And Methods: The study was carried out in 44 municipalities in the department of Antioquia, Colombia, in 2003 and 2004, with a randomly selected sample of 1 624 rural and urban households with children under 10 years of age, representative for family units located in the department of Antioquia. The sample was selected using a confidence interval of 95% and an error of 3%.