Pulse labeling of dissolved (13) C-carbonate into tree xylem: developing a new method to determine the fate of recently fixed photosynthate.

Stable carbon isotopes are often employed as tracers in plant and soil systems to study the fate and transformations of carbon as is it assimilated by the forest canopies and then translocated into the soil matrix and soil microorganisms. This experiment tested a new method of (13) C-labeling. We dissolved (13) C-carbonate into 12 mL of water and injected it into the xylem of a 6-cm diameter tree.