Development of a Single-Piece Sperm Counting Chamber (SSCC) for Aquatic Species.

Accurate determination of sperm concentration in aquatic species is important for assisted reproduction and cryopreservation, yet is challenging as current counting methods are costly or not suitable for many species. The goal of this work was to develop a simple (single-piece and single-layer photolithography) sperm counting chamber (SSCC) for aquatic species. Goldfish ( and zebrafish () sperm were used for evaluation in the device, which was created with soft lithography.