Publications by authors named "Elizabeth J Anthony"
Article Synopsis
- Mammalian DNA replication requires various helicases and nucleases for accurate genetic duplication, but the direction of these activities was previously unclear.
- The study identifies USP50 as a crucial chromatin-associated protein that aids in ongoing replication, fork restart, and telomere maintenance, while also preventing DNA breaks.
- USP50 works by ensuring the correct localization of other proteins like WRN and FEN1 during stalled replication, and its absence leads to increased activity of certain helicases and nucleases, causing replication issues and telomere instability.
A synthetic lethal relationship exists between disruption of polymerase theta (Polθ), and loss of either 53BP1 or homologous recombination (HR) proteins, including BRCA1; however, the mechanistic basis of these observations are unclear. Here we reveal two distinct mechanisms of Polθ synthetic lethality, identifying dual influences of 1) whether Polθ is lost or inhibited, and 2) the underlying susceptible genotype. Firstly, we find that the sensitivity of BRCA1/2- and 53BP1-deficient cells to Polθ loss, and 53BP1-deficient cells to Polθ inhibition (ART558) requires RAD52, and appropriate reduction of RAD52 can ameliorate these phenotypes.
View Article and Find Full Text PDFMetals play vital roles in nutrients and medicines and provide chemical functionalities that are not accessible to purely organic compounds. At least 10 metals are essential for human life and about 46 other non-essential metals (including radionuclides) are also used in drug therapies and diagnostic agents. These include platinum drugs (in 50% of cancer chemotherapies), lithium (bipolar disorders), silver (antimicrobials), and bismuth (broad-spectrum antibiotics).
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