Clinical consequences of an increasing trend of preferential use of cultured villi for molecular diagnosis by CVS.

Objective: To compare the use of uncultured versus cultured villus cells for DNA-based prenatal diagnosis.

Methods: A retrospective review of molecular testing of chorionic villus sampling (CVS) cases from 1988-2007. Method of analysis, gestational age (GA) at CVS and at diagnosis, time from procedure to results, results of maternal contamination studies, and the laboratory employed were abstracted from patient charts.