Lysine acetylation of aquaporin-3 promotes water permeability but is not essential for urine concentrating ability.

Aquaporin-3 (AQP3) mediates basolateral water transport in the kidney principal cells contributing to urine concentration. We previously identified the acetylation of lysine 282 (K282) in the C-terminus of AQP3, which we hypothesized as a positive regulator of AQP3 water permeability. AQP3 acetylation (K282Q or Q), or deacetylation (K282R or R) mimetic mutant mice models were created using CRISPR/Cas9.