Publications by authors named "Elizabeth A Rubie"
Article Synopsis
- Glycogen synthase kinase 3 (GSK3) inhibits myogenic differentiation and myoblast fusion by blocking the Wnt/β-catenin signaling pathway; lithium can reduce GSK3 activity.
- In this study, a low dose of lithium (0.5 mM) was shown to enhance myoblast fusion and differentiation in muscle cells, which is significant for muscle health.
- Treatment with 0.5 mM lithium resulted in increased phosphorylation of GSK3, reduced GSK3 activity by 86%, and higher levels of myogenic markers, indicating its potential therapeutic effects for muscle-related disorders.
Article Synopsis
- * In triple-negative breast cancer (TNBC), RB1 is often inactivated alongside PTEN and TP53, leading researchers to conduct inhibitor screens on cell lines that lack these tumor suppressors.
- * The study identified CDC25 phosphatase as a promising therapeutic target, showing that inhibiting CDC25 can suppress the growth of RB1-deficient TNBC cells, especially when combined with WEE1 and PI3K inhibitors.
The prion protein (PrP) evolved from the subbranch of ZIP metal ion transporters comprising ZIPs 5, 6 and 10, raising the prospect that the study of these ZIPs may reveal insights relevant for understanding the function of PrP. Building on data which suggested PrP and ZIP6 are critical during epithelial-to-mesenchymal transition (EMT), we investigated ZIP6 in an EMT paradigm using ZIP6 knockout cells, mass spectrometry and bioinformatic methods. Reminiscent of PrP, ZIP6 levels are five-fold upregulated during EMT and the protein forms a complex with NCAM1.
View Article and Find Full Text PDFThe highly selective glycogen synthase kinase-3 (GSK-3) inhibitor N-(4-methoxybenzyl)-N'-(5-nitro-1,3-thiazol-2-yl)urea (AR-A014418) was radiolabeled with carbon-11 ((11)C; half-life=20.4min) at the urea moiety via [(11)C]CO(2) fixation. Reaction of [(11)C]CO(2) with 4-methoxybenzylamine in the presence of a CO(2) fixating base was followed by dehydration with POCl(3) and addition of 2-amino-5-nitrothiazole to prepare [(11)C-carbonyl] AR-A014418.
View Article and Find Full Text PDFProtein kinase A (PKA) is an important signal transduction target for drug development because it influences critical cellular processes implicated in neuropsychiatric illnesses such as major depressive disorder. The goal of the present study was to develop the first imaging agent for measuring the levels of PKA with positron emission tomography (PET). By rational derivatization of 5-isoquinoline sulfonamides, it was found that the introduction of a methyl group to the sulphonamidic nitrogen on the known PKA inhibitors N-(2-aminoethyl)isoquinoline-5-sulfonamide (H-9, 1) and N-(2-(4-bromocinnamylamino)ethyl)isoquinoline-5-sulfonamide (H-89, 2), (yielding N-(2-aminoethyl)-N-methyl-isoquinoline-5-sulfonamide (4) and N-(2-(4-bromocinnamylamino)ethyl)-N-methyl-isoquinoline-5-sulfonamide (5), respectively) does not appreciably reduce in vitro potency toward PKA.
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