Lipid Composition of M12 Strain Grown on Glycerol as a Carbon Source.

Research Background: In this study the content and composition of lipids in ergosterol-reduced M12 strain grown on glycerol as a carbon source is determined. Blocking the ergosterol synthesis route in yeast cells is a recently proposed method for increasing -adenosyl-l-methionine (SAM) production.

Experimental Approach: The batch cultivation of M12 yeast was carried out under aerobic conditions in a laboratory bioreactor with glycerol as carbon source, and with pulsed addition of methionine.